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Purification of mevalonate from fermentation broth and conversion of biomevalonate into biomevalonolactone

초록/요약

Mevalonate (MVA) is a key compound of living organisms including bacteria, plants, and humans. MVA and mevalonolactone (MVL), a lactonized form of MVA, are important for pharmaceutical, cosmeceutical, and biotechnological applications. Although (R, S)-MVA with 50% enantiomeric purity is mainly produced by chemical synthesis, recently, microbial fermentation processes for MVA production have been considered as an alternative to the chemical synthesis because of high enantiomeric purity [(R)-MVA] and high titer. In the present study, bio-MVA produced by a fermentative process was decolorized by a charcoalbased method and then chemically transformed into bio-MVL without byproducts by means of phosphoric acid as an acid catalyst. The final bio-MVL was (R)-MVL with over 99% enantiomeric purity according to 1H NMR analysis.

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목차

1. Introduction 1
2. Materials and methods 3
2.1 Bacterial strains and plasmids 3
2.2 Media and culture condition 5
2.3 Pilot scale fermentation 5
2.4 MVL purification process 7
2.5 NMR analysis 7
3. Results 8
3.1 MVA production 8
3.2 Bioconversion of MVA into MVL and purification of MVL 8
4. Conclusion 13
5. Reference 14

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