Development of Theragnostic Tool Using NIR Fluorescence Probe Targeting Mitochondria in Glioma Cells
- 주제(키워드) NIR , Mitochindria , Glioblastoma , Theragnosis
- 발행기관 아주대학교
- 지도교수 김은하
- 발행년도 2020
- 학위수여년월 2020. 8
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000030292
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Chemical biology is a scientific discipline between chemistry and biology. It provides various way to explore biological phenomena in the cellular level and involves the application of chemical techniques, analysis, and often small molecules produced through synthetic chemistry, to the study and manipulation of biological systems. In this study, we develop new theragnostic tools targeting mitochondria in Glioma cells. Because mitochondria are essential organelles for regulating energy homeostasis and intrinsic apoptosis, the perturbation of mitochondrial functions has been considered as an anti cancer treatment. Therefore, a new near-infrared (NIR) fluorescent probe, SiR-Mito11 targeting mitochondria was developed as a theragnostic agent and then was applicable Chemical biology is a scientific discipline between chemistry and biology. It provides various way to explore biological phenomena in the cellular level and involves the application of chemical techniques, analysis, and often small molecules produced through synthetic chemistry, to the study and manipulation of biological systems. In this study, we develop new theragnostic tools targeting mitochondria in Glioma cells. Because mitochondria are essential organelles for regulating energy homeostasis and intrinsic apoptosis, the perturbation of mitochondrial functions has been considered as an anti cancer treatment. Therefore, a new near-infrared (NIR) fluorescent probe, SiR-Mito11 targeting mitochondria was developed as a theragnostic agent and then was applicable for brain tumor models. SiR-Mito11 exhibited a potential anti-cancer activity against glioma cells, but was tolerant in normal neuronal cells. We further confirmed that the selective accumulation of SiR-Mito11 in glioma cells disrupted mitochondria membrane potential, followed by apoptotic cell death. In conclusion, through this approach, it is possible to treat various brain tumor. This study will contribute to understanding of the complex biological phenomena for targeted therapies.
more목차
1. Introduction 1
2. Results and discussions 4
2.1 Theragnostic near-infrared mitochondrial probe, SiR-Mito11 4
2.2 Cancer cell selective toxicity of SiR-Mito11 6
2.3 Different cellular uptake of SiR-Mito11 in normal and cancer cells 9
2.4 Disruption of mitochondria membrane potential by SiRMito11 in HT22 and H4 cells 11
2.5 SiR-Mito11 selectively induced apoptosis in H4 cells 12
3. Materials and methods 16
4. Conclusion 20
Reference 21
