Genome Sequencing and Carotenoid Pathway Analysis of Newly Isolated Deinococcus sp. AJ005
- 주제(키워드) Deinococcus , deinoxanthin
- 발행기관 아주대학교
- 지도교수 이평천
- 발행년도 2020
- 학위수여년월 2020. 8
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000030084
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Our purpose was to characterize the structures of deinoxanthin from Deinococcus sp. AJ005. The latter is a novel reddish strain and was found to synthesize two main acyclic carotenoids: deinoxanthin and its derivative. The derivative (2-keto-deinoxanthin) contains a 2-keto functional group instead of a 2-hydroxyl group on a β-ionone ring. A deinoxanthin biosynthesis pathway of Deinococcus sp. AJ005 involving eight putative enzymes was proposed according to genome annotation analysis and chemical identification of deinoxanthin. Optimal culture pH and temperature for Deinococcus sp. AJ005 growth were pH7.4 and 20°C. Sucrose as a carbon source significantly enhanced the cell growth in comparison with glucose, glycerol, maltose, lactose, and galactose. When batch fermentation was performed in a bioreactor containing 40g/L sucrose, total carotenoid production was 650% higher than that in a medium without sucrose supplementation. The culture conditions found in this study should provide the basis for the development of fermentation strategies for the production of deinoxanthin and of its derivative by means of Deinococcus sp. AJ005.
more목차
1. Introduction 1
2. Materials and Methods 3
2.1 Genome sequencingof Deinococcus sp. AJ005 3
2.2 Flask fermentation involving Deinococcus sp. AJ005 5
2.3 Bioreactor fermentation by Deinococcus sp. AJ005 5
2.4 Extraction and analysis of carotenoids 6
2.5 Monitoring cell growth and quantification of carbohydrates and Carotenoids 7
3. Results and Discussion 9
3.1 Genome sequencing of Deinococcus sp. AJ005 9
3.2 Characterization of carotenoids of Deinococcus sp. AJ005 13
3.3 The proposed carotenoid biosynthetic pathway and genes encoding putative carotenogenic enzymes in Deinococcussp. AJ005 17
3.4 Effects of culture media, culture pH, and temperature on cell growth 21
3.5 Batch fermentation involving Deinococcus sp. AJ005 for carotenoid production 24
4. Conclusion 26
5. References 28
