The Polarization of Human Gingival Fibroblasts by T Helper Cytokines
T helper 사이토카인에 의한 인간 치은 섬유아세포의 분극화
- 주제(키워드) gingival fibroblast , periodontitis , inflammation , T helper cytokine , polarization
- 발행기관 아주대학교
- 지도교수 지 숙
- 발행년도 2020
- 학위수여년월 2020. 8
- 학위명 석사
- 학과 및 전공 임상치의학대학원 임상치의학과
- 실제URI http://www.dcollection.net/handler/ajou/000000030042
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Periodontitis is an inflammatory disease against periodontal pathogen that results in loss of periodontal attachment and marginal alveolar bone destruction . It is character ized by infiltration of several immune cell types . S ubsets of CD4 T cells such as, T helper 1 (Th1), Th2, Th17 and T regulatory (Treg) cells have been found in diseased gingival tissues. Gingival fibroblasts (GFs) are predominant cell type in gingival conn ective tissue and it has been proven for being an essential regulatory function in the progression of periodontitis by inflammatory response and extracellular matrix remodeling . The aim of this study was to evaluate whether GFs can be polarized into functi onal ly different subtypes by Th cytokines. Cultured human GFs were stimulated by 4 cytokines of Th1 (interferon gamma (IFN γ )), Th2 (interleukin 4 (IL 4)), Th17 (interleukin 17 (IL 17)) and regulatory T (transforming growth factor beta (TGF β )) cytokines. Messenger RNA expression profiling was analyzed by microarray. Several genes that appeared to be highly up or down regulat ed in the microarray analysis were selected for reverse transcription polymerase chain reaction (RT-PCR). Up regulation and down regulation of genes on GFs by IFN γ was most prominent in absolute maximum fold change value and the number of expressed probes . The pattern of genetic expression on GFs by 4 Th cytokines (IFN-γ, IL-4, IL-17 or TGF-β) was shown functionally different. IFN-γ and IL-17 up-regulated genes related to inflammatory responses and extracellular matrix destruction whereas down-regulated genes related to anti-inflammatory responses and matrix synthesis. IL-4 promoted the expression of anti-inflammatory or tissue-reparative genes while suppressed inflammatory or tissue-destructive genes. TGF-β usually functioned like IL-4 up-regulating the expression of anti-inflammatory genes but also induced the expression of several inflammatory genes, such as pro-inflammatory cytokines. In conclusion, GFs can be polarized. In conclusion, GFs can be polarized into functionally different into functionally different subtypes by Th cytokines, and thus can function to promote or suppress inflammation and tissue destruction in gingival connective tissue.
more목차
I. INTRODUCTION 1
II. MATERIALS AND METHODS 6
A. MATERIALS 6
B. METHODS 7
III. RESULTS 12
IV. DISCUSSION 32
V. CONCLUSION 39
REFERENCES 40
국문요약 49
