알츠하이머 모델의 뇌 허혈 시 세포 사멸에 대한 취약성
Ischemic Vulnerability in the Brain of an Alzheimer’s Mouse Model
- 주제(키워드) Alzheimer's disease , Brain ischemia
- 발행기관 아주대학교
- 지도교수 이진수
- 발행년도 2019
- 학위수여년월 2019. 2
- 학위명 석사
- 학과 및 전공 일반대학원 의생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000028838
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Alzheimer's disease (AD) is a condition that mostly affects elderly individuals who are also at an increased risk of cerebrovascular disease. It is not well known if AD pathology results in poorer outcomes for cerebral ischemia. In the current study, we hypothesized that a brain affected by AD is vulnerable to cerebral ischemia, resulting in greater necrosis and neuronal cell death by inducing cerebral amyloid beta (Aβ) and tau protein accumulation, as well as degenerative changes. In this study, the effect of transient cerebral ischemia induced by bilateral carotid artery occlusion, which is often used as a vascular dementia model, was assessed in a transgenic (TG) mouse model of AD (3xTG-AD). After 15 minutes of bilateral carotid artery occlusion, followed by reperfusion, we evaluated cognitive changes, extent of neuronal death, and dementia-specific neuropathological changes compared with wild-type (WT) mice. Results of behavioral studies demonstrated that TG mice exposed to cerebral ischemia showed greater deficits in spatial learning and memory than similarly treated WT mice. Cognitive impairment in TG mice might be attributed to greater neuronal cell death due to brain ischemia compared with WT mice. However, similar Aβ accumulation was observed in both groups. Expression of caspase-3, Iba-1, and GFAP was increased in ischemic TG mice. In conclusion, our results suggest that Alzheimer’s neuropathology can trigger changes that make AD brains more vulnerable to cerebral ischemia/reperfusion.
more목차
I. INTRODUCTION
II. MATERIALS AND METHODS
A. Animal care and study time frame
B. Surgery
C. Behavior tests
1. Morris water maze test
2. Y maze test
D. Tissue processing
E. Cresyl violet staining
F. Immunohistochemistry
G. Immunofluorescence staining
H. Thioflavin S
I. Statistical analyses
III. RESULTS
A. Behavior testing after tBCCAO
B. Neuronal cell death
C. Changes in Alzheimers pathology
1. Amyloid plaques
2. Amyloid precursor protein
3. Tau protein
D. Glial changes
IV. DISCUSSION
V. REFERENCES
국문요약
