감초와 닥나무의 촉매진공추출과 수상스틱을 이용한 사전 미백 평가
Catalytic vacuum extraction of Glycyrrhiza and Broussonetia, and preliminary evaluation of skin-whitening using water stick
- 주제(키워드) Skin-whitening , Tyrosinase , Hydrogel , Glycyrrhiza , Broussonetia
- 발행기관 아주대학원
- 지도교수 이범진
- 발행년도 2019
- 학위수여년월 2019. 2
- 학위명 석사
- 학과 및 전공 일반대학원 의용공학과정
- 실제URI http://www.dcollection.net/handler/ajou/000000028586
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
In this study, Glycyrrhiza and Broussonetia were extracted with hot water, 50% ethanol and vacuum catalyst extraction method to confirm skin-whitening effect of Glycyrrhiza and Broussonetia which are effective for whitening. The inhibitory activity of tyrosinase and the production rate of melanin pigment were evaluated for evaluation(In Vitro) of the whitening effect of each extract. As a result of the experiment, 50%-ethanol extracts showed the best whitening effect at the same concentration. In addition, the efficacy of a 1:1 blend of each component was marginally better than that of each component. The extracts of vacuum extraction method were relatively low in whitening effect, but these extracts showed very low toxicity in cytotoxicity test (MTT assay). Even at a sample concentration of 20%, the cell activity was slightly increased. As a result, since the extracts of Glycyrrhiza and Broussonetia can be presumed to be 10 to 20 times higher in content, it is possible to obtain a prescription with sufficient whitening effect using a high concentration of extract. Based on the efficacy evaluation experiments, formulation was achieved by using 20% Glycyrrhiza and Broussonetia extracts. On the other hand, many formulations such as milky lotions, creams, and mask packs have already been subjected to many experiments and many whitening evaluations have been conducted. Therefore, in order to expand the application of whitening raw materials, effective extracts were added to the Water stick (Water dispersion stick fomulation.), which is a specific formulation. Since the common wax raw material is hydrophobic, hydrogel network is formed at high density using carrageenan to realize hydrophilic extracts as a stick formulation, and stick formulation is implemented by correcting the hardness with a solid surfactant, sodium stearate. Samples prepared by the experiment were photographed with a scanning electron microscope (FE-SEM) to confirm that the gel network was formed at a high density, and the effectiveness of the whitening function was finally verified through human application experiments.
more목차
Ⅰ. Introduction 1
A. Whitening cosmetics and melanin pigment 1
B. Melanin pigment 2
C. Tyrosinase and Skin-Whitening 6
D. Glycyrrhiza and Broussonetia 7
E. Water stick and clinical effect 8
Ⅱ. Materials and Methods 10
A. Materials 10
B. Extraction method of Glycyrrhiza and Broussonetia 10
C. In vitro Tyrosinase inhibition assay 11
D. Cell and tissue culture 12
E. MTT assay of Glycyrrhiza and Broussonetia extracts 12
F. Melanin synthesis inhibition test 13
G. Water stick fomulation experiment 14
H. Clinical Test of skin-whitening 16
Ⅲ. Results and Discussion 18
A. MTT assay of Glycyrrhiza and Broussonetia extracts 18
B. In vitro Tyrosinase inhibition assay 20
C. Melanin synthesis inhibition test 20
D. Water stick fomulation experiment 23
E. Clinical Test of skin-whitening 23
Ⅳ. Conclusions 29
REFERENCES 31
