갈근발효추출물의 주름개선 및 피부 전달 연구
Study of Wrinkle Improvement and Skin Delivery of Fermented Pueraria lobata Ohwi Extracts
- 주제(키워드) Pueraia lobata Ohwi , Fermentation , Antioxidant , Liposome , Absorption
- 발행기관 아주대학교
- 지도교수 이범진,김수동
- 발행년도 2019
- 학위수여년월 2019. 2
- 학위명 석사
- 학과 및 전공 일반대학원 응용생명공학과
- 실제URI http://www.dcollection.net/handler/ajou/000000028580
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Cosmetics are changing to the concept of functional effect such as whitening, wrinkle improvement, and ultraviolet ray shielding in a basic concept that adds charm to the human body and changes the appearance brightly. In this study, the antioxidative and wrinkle - improving effects of natural materials, which have excellent antioxidative and safety properties, are investigated. The antioxidant activity of Pueraria lobata Ohwi extracts was confirmed by DPPH assay and total polyphenol content and total flavonoid content were measured. In order to increase antioxidant activity of Pueraria lobata Ohwi extracts, Lactobacillus Fermentum MieV L1208, which was commissioned by M&C Life Science, showed the highest antioxidative effect of Pueraria lobata Ohwi extracts after the fermentation. It was confirmed that the fermented extracts of Pueraria lobata Ohwi were confirmed to have no cytotoxicity by 20% using fibroblast HDF cells, and the activities of inhibition of intracellular collagenase and elastase activity were inhibited compared to the control group. The results of liposomalization of fermented Pueraria lobata Ohwi extracts with antioxidant activity and wrinkle - improving effect were compared with skin absorption by using Franz Diffusion Cell. The liposomalization of fermented Pueraria lobata Ohwi extracts showed increased absorption capacity. As a result, it was confirmed that the size of liposomal Pueraria lobata Ohwi extracts was reduced to about 250 nm and it had uniform distribution. As a result of observing through electron microscope, it was found that a stable spherical shape was formed Based on the above results, it was confirmed that the fermeted Pueraria lobata Ohwi extracts showed high antioxidant ability, and it could help to improve wrinkles and skin elasticity, and the liposomized fermented Pueraria lobata Ohwi extracts is highly likely to be used as a cosmetic material having excellent efficacy.
more목차
Ⅰ. Introduction 1
Ⅱ. Materials and Methods 4
A. PF Manufacturing Methods 4
B. Identification of Antioxidant and cytotoxicity 5
1. DPPH Assay 5
2. Total Polyphenol 5
3. Total Flavonoid 6
4. MTT Assay 6
C. Wrinkle improvement experiments 7
1. Inhibition effects on collagenase activity 7
2. Inhibition effects on Elastase activity 8
D. Analysis and absorption measurements 9
1. PE4 Manufacturing Methods 9
2. Morphological analysis 11
3. Absorption force measurement 11
Ⅲ. Results and Discussion 13
Ⅳ. Conclusions 26
REFERENCES 28
