양배추 배추에 접촉한 살모넬라의 전반적인 전사체반응에 대한 이해
Understanding comprehensive transcriptional response of Salmonella enterica spp. in contact with cabbage and napa cabbage
- 주제(키워드) Salmonella , vegetable , transcriptome , virulence
- 발행기관 아주대학교
- 지도교수 윤현진
- 발행년도 2018
- 학위수여년월 2018. 8
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000027934
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
An increasing number of foodborne diseases are currently attributable to fresh vegetables and fruits contaminated with enteric pathogens such as Salmonella enterica spp. and Escherichia coli. Recent studies demonstrate that a variety of enteric pathogens are able to colonize onto plant surfaces and persist for a long time, which can subsequently lead to human infections. In order to understand the underlying mechanism of Salmonella adaptation to plants, I investigated the transcriptomics of Salmonella in contact with green vegetables cabbage and napa cabbage. Interestingly, Salmonella pathogenicity island (SPI)-1 genes, which are required for Salmonella invasion into host cells, were up-regulated upon contact with vegetables, suggesting that SPI-1 may be implicated in Salmonella colonization onto plant tissues as well as animal tissues. Furthermore, Salmonella transcriptomic profiling provided several genetic loci that showed significant changes in their expression in response to vegetables and were associated with bacterial adaptation to hostile niches, including nadA, fdnGHI, speF/potE, STM14_2269, ogt, and cbi operon. nadA was required for bacterial growth under nutrient-restricted conditions, while the others were required for bacterial invasion into host cells. The transcriptomes of Salmonella in contact with cabbage and napa cabbage provided insights into the comprehensive bacterial transcriptional response to plants and also suggested diverse virulence determinants relevant to Salmonella survival and adaptation.
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CONTENTS
ABSTRACT ⅰ
LIST OF TABLES ⅳ
LIST OF FIGURES ⅴ
1. Introduction 1
2. Materials and Methods 3
2.1 Bacterial strains and growth condition 3
2.2 Preparation of cabbage and napa cabbage 12
2.3 RNA extraction 12
2.4 RNA-Sequencing and analysis 12
2.5 Quantitative real-time PCR analysis 13
2.6 Biofilm assay 13
2.7 Invasion assay 14
2.8 Motility assay 14
2.9 ROS test 14
3. Results 15
3.1 Effect of cabbage and napa cabbage on growth of Salmonella 15
3.2 Comprehensive transcriptomic analysis of Salmonella enterica spp. upon contact with cabbage and napa cabbage 17
3.3 Differential regulation of Salmonella virulence determinants in response to cabbage and napa cabbage 19
3.4 Defining genes differentially expressed in contact with cabbage and napa cabbage 21
3.5 Exploring genes associated with Salmonella adaption into hostile environments 25
3.6 Understanding roles of cbi operon as a virulence determinant in Salmonella 30
4. Discussions 33
5. Reference 36
6. Abstract in Korea 41
