Non-thermal plasma inhibits mast cell activation and ameliorates allergic skin inflammatory diseases in NC/Nga mice.
- 주제(키워드) Non-thermal plasma(NTP) , Atopic dermatitis , Mast cell , Allergic skin disease
- 발행기관 아주대학교
- 지도교수 김철호
- 발행년도 2018
- 학위수여년월 2018. 2
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000027298
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Non-thermal plasma (NTP) has many functional activities such as, sterilization, wound healing and anti-cancer activity. Thus, its application in bio-medical field is expanding quickly. However, the effect of NTP on immune cells and allergic response has not been well studied. In this study, we determined whether NTP could suppress mast cell activation, which is important for allergic response, and ameliorate an atopic dermatitis (AD)-like skin inflammatory disease in mice. Treatment of NTP-treated medium during mast cell activation inhibited IL-6, TNF-α expression and production and suppressed NF-κB activation. We also investigated whether NTP treatment could ameliorate house dust mite (HDM)-induced AD-like skin inflammation in mice. NTP treatment inhibited increases in epithermal thickness and recruitment of mast cells and eosinophils, which are important cell types for the pathogenesis of AD. In addition, Th2 cell differentiation was induced by application of HDM and the differentiation was also inhibited in the draining lymph node of NTP-treated mice. Finally, the expression of AD-related cytokines and chemokines also decreased in NTP-treated mice. Taken together, these results suggest that NTP might be useful in the treatment of allergic skin diseases, such as AD.
more목차
Ⅰ. Introduction 1
Ⅱ. Material and Method 4
A. Physical properties of N2 plasma and N2 plasma device 4
B. Generation and treatment of LTP 4
C. Cell line and reagents 5
D. RNA isolation, complementary DNA synthesis and quantitative real-time PCR analysis 5
E. Western blot analysis 6
F. Mice 6
G. HDM-induced AD in mice 7
H. Immunohistochemistry 7
I. Generation of mouse bone marrow-derived mast cells 8
J. Flow cytometry and intracellular cytokine analysis 8
K. Statistical analysis 9
Ⅲ. Results 10
A. LTP treatment inhibits mast cell activation 10
B. NTP treatment ameliorates HDM-induced AD-like skin inflammation in mice 11
C. NTP treatment inhibits Th2 cell differentiation and AD- related gene expression in mice 12
D. LTP treatment inhibits the inflammatory response in HaCaT 13
Ⅳ. Discussion 26
Ⅴ. Reference 29
국문요약 35
