골형성 단백질과 정전기적인 인력을 형성하는 하이드로겔에서 인간 비갑개 중간엽 줄기세포의 생체내 골분화
In vivo osteogenic differentiation of human turbinate mesenchymal stem cells in hydrogel forming electrostatic attraction with bone morphogenetic proteins-2
- 주제(키워드) human turbinate mesenchymal stem cells , osteogenic differentiation , electrostatic attraction , thermo-responsive hydrogels
- 발행기관 아주대학교
- 지도교수 김문석
- 발행년도 2018
- 학위수여년월 2018. 2
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000027124
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Currently tissue engineering can solve the problem of shortage of donated organs using regenerative techniques of artificial skin, cartilage, bone, bladder, myocardium, liver, etc. In this study, we examined the osteogenic differentiation of human turbinate mesenchymal stem cells (hTMSCs) in vivo by using injectable hydrogel forming electrostatic attraction with bone morphogenetic proteins-2 (BMP2). hTMSCs are easily isolated from inferior turbinate tissue, exhibited abundant and proliferated at a high rate. Also hTMSCs have the ability to differentiate into osteoblasts. Methoxy polyethylene glycol-b-poly(caprolactone)-ran-(3-benzyloxymethyl lactide) diblock copolymer (PCL-Bz) were derivatized by introducing a pendant amine group (PCL-NH2) at the benzyl group. The amine group of PCL-NH2 forms the electrostatic attraction with BMP-2 having a negative charge. Electrostatic attraction reduces initial burst release and increases stability of BMP-2 in PCL-NH2 hydrogel. So we injected PCL-NH2 hydrogel and poly(caprolacton)-ran-(lactide) (PCL) hydrogel which does not make electrostatic attraction with BMP2 into the mice subcutaneous to confirm the bioavailability of BMP-2 by electrostatic attraction. Osteogenic differentiation of hTMSCs was identified by von Kossa (VK) and alizarin red S (ARS) staining of removed hydrogels at 2, 4, 8 weeks and osteonectin, osteopontin, osteocalcin, and collagen type 1α mRNA expression. As a result, PCL-NH2 hydrogel has a slower drug release and more bone formation than PCL hydrogel. In conclusion, PCL-NH2 hydrogel that form electrostatic attraction with growth factors and drugs will be used in many applications in tissue engineering.
more목차
1. Introduction 1
2. Experimental 5
3. Results 16
4. Conclusion 43
5. References 44
APPENDIX 52
ABSTRACT (IN KOREAN) 55
