Dopa-Fe3+ 결합과 펜톤 반응을 통한 이중가교 결합된 젤라틴 기반 하이드로젤의 합성 및 특성분석
Dual-crosslinked Gelatin Hydrogels Comprising Dopa-Fe3+ Complexation and Fenton Reaction ; Synthesis and Characterization
- 주제(키워드) dopamine , Fenton reaction , dual crosslinking , gelatin , and Dopa-Fe3+ coordination complexation
- 발행기관 아주대학교
- 지도교수 박기동
- 발행년도 2017
- 학위수여년월 2017. 8
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000025985
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
A dual-crosslinked gelatin hydrogel via Dopa-Fe3+ complexation and Fenton reaction was developed as a hydrogel fabrication method to prepare dual-crosslinked hydrogels for biomedical application. To prepare the dual-crosslinked hydrogels in PBS (pH 7.4), the acryl moieties at the gelatin conjugate were firstly crosslinked via Fenton reaction and created Fe3+ ions as a byproduct. These Fe3+ ions used to form Dopa-Fe3+ complexations as a second crosslinking. The hydrogel was formed rapidly using hydrogen peroxide (H2O2) and Iron(Ⅱ) chloride (FeCl2). The mechanical properties could be controlled by varying the H2O2 concentration. The mechanical strength measurement results clearly showed that the methacrylated gelatin dopamine (GMD) hydrogels exhibited 2 or 4 times higher storage moduli compared to the methacrylated gelatin (GMA) or gelatin dopamine (GDA) hydrogels due to dual crosslinking. This hydrogel may be applied as a variety of tissue sealants because of its adhesion that dopamine moieties induce. This newly developed GMD hydrogels don’t use oxidizing agents in mild condition and have great potential for use as injectable materials in tissue regenerative medicine and various biomedical applications.
more목차
Ⅰ. INTRODUCTION 1
1. Tissue engineering 1
1.1. Basic elements in TE 2
1.2. Hydrogel as scaffold 4
1.2.1. Hydrogel forming materials 5
1.2.2. Crosslinking methods for fabricating hydrogels 6
1.3. Dopamine moieties as biomimetic approach 8
1.3.1. Properties and origins of dopamine 8
1.3.2. Current studies and application about dopamine as materials 10
1.3.3. Various method of preparing dopamine-conjugated hydrogels 12
2. Objectives 18
Ⅱ. EXPERIMENTS 20
1. Materials 20
2. Synthesis and characterization of methacrylated gelatin dopamine conjugates 20
3. Preparation of hydrogels 22
4. Rheological measurement and swelling ratio 23
5. In vitro proteolytic degradation 23
6. Stability of hydrogels 23
7. Tissue adhesive strength 24
Ⅲ. RESULTS AND DISCUSSIONS 25
1. Synthesis and characterization of polymers 25
2. Rheological analysis and swelling ratio of hydrogels by varying H2O2 concentration 27
3. Proteolytic degradation behaviors 28
4. Stability test in the simulated body fluid 29
5. Effect of dopamine on adhesive strength of hydrogels 31
Ⅳ. CONCLUSION 33
Ⅴ. REFERENCE 34
