목차

I. INTRODUCTION 1
1. Cell Junctions among the Adjacent Cells 1
2. The Role of Nectin Family 2
3. Molecular Structure of Nectin-2 3
4. The Diverse Function of Nectin-2 4
II. MATERIALS AND METHODS 9
1. Cell Culture and Cell Line 9
2. Monoclonal Antibody Generation 9
3. Indirect ELISA 10
4. RNA Extraction and cDNA Synthesis 11
5. Cloning of Variable Region in Monoclonal Antibody 12
6. Surface Plasma Resonance Analysis 13
7. FACS Analysis 13
8. Immunoprecipitation Assay and Western Blot 14
9. RNA Interference 15
10. RT-PCR and qRT-PCR 16
11. Statistical Analysis 16
III. RESULTS 21
1. Generation of Monoclonal Nectin-2 Antibody 21
2. Characterization of Nectin-2 Mouse Monoclonal Antibody 33
3. The Expression of Nectin-2 in Cell Lines 37
IV. DISCUSSION 43
V. CONCLUSION 47
VI. REFERENCES 48
VII. ABSTRACT IN KOREAN 55