Studies on skin moisturizing and anti-inflammatory efficacy of peptide and amino acid hydrolysate from ginseng seed
Studies on skin moisturizing and anti-inflammatory efficacy of peptide and amino acid hydrolysate from ginseng seed
- 주제(키워드) Ginseng seed protein , peptides , amino acids , Moisturizing , Anti-inflammation.
- 발행기관 아주대학교
- 지도교수 Sang Yo Byun
- 발행년도 2017
- 학위수여년월 2017. 8
- 학위명 석사
- 학과 및 전공 일반대학원 응용생명공학과
- 실제URI http://www.dcollection.net/handler/ajou/000000025640
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
The aim of this study was to determine skin moisturizing and anti-inflammatory efficacy of complex peptide & amino acid hydrolysate derived from ginseng seed protein. It’s believed that ginseng seeds contain a variety of functional and bioactive protein. Ginseng seed protein was extracted 18.9% of yields from defatted Korean Panax ginseng C.A. Meyer seeds. In order to solve transdermal problem of protein and enhance skin penetration, then the protein was digested by a parallel combination of bromelain and exo-peptidase as protease to produce small molecular peptides and amino acids in the hydrolysate. They are easy to penetrate into skin. The resultant hydrolysate was comprised by 36% amino acids and around 60% small peptide (<1000Da). After purifying, the hydrolysate from ginseng seed protein was investigated on efficacy test of skin moisturizing and anti-inflammation. The study showed moisture contents in stratum corneum(SC) increased around 20% over 5hrs of sample application. NMFs contents increased 51% and lipid contents increase 20% more than control in SC. Furthermore, proteomic SDS-PAGE analysis of HaCaT cells indicated complex peptide and amino acid hydrolysate up-regulated expression of the protein related to 3 moisturizing mechanisms, such as JAM-1 upped to 831%, involucrin upped to 1573%, AQP-3 upped to 519%. In addition, the 3000ppm of complex peptide and amino acid hydrolysate inhibited 60% nitric oxide (NO) production from LPS-induced RAW 264.7 cell. In the further study, some key inflammatory “messenger” protein in the RAW 264.7 cell had significant down regulation because of the complex hydrolysate. Such as TLR4 downed to 35%, IRAK4 and Ikkα downed to 15.2% and 4.1% respectively et al. The results demonstrated complex peptide & amino acid hydrolysate from ginseng seed protein is a potential active ingredient that can be applied for skin moisturizing and anti-inflammation efficacy in the cosmetics.
more목차
I.INTRODUCTION 1
A.Enzymatic hydrolysate derived from seed 1
B.Skin hydration mechanisms 4
C.Inflammation mechanisms 9
D.Purpose of research 12
II.MATERIALS & METHODS 13
A.Materials & Instruments 13
B.Methods 14
1.Protein extraction of ginseng seed 14
2.Hydrolysis of ginseng seed protein 14
3.Efficacies investigation 15
3.1Skin water holding capacity and NMFs-Lipid evaluation 15
3.2Inhibition of NO(Nitric oxide) production assay 17
3.3Two dimensional electrophoreses 18
III.RESULT & DISCUSSIONS 20
A.Complex peptide and amino acid hydrolysate from ginseng seed protein 20
1.Protein extraction from ginseng seeds 20
2.Preparation and optimization of hydrolysate from ginseng seed protein 23
B.Results on skin moisturizing effect of complex hydrolysate from ginseng seed protein 28
1.Skin water holding capacity and NMFs-Lipid evaluation 28
2.Hydration test of human keratinocyte (HaCaT) cell via proteomic analysis 36
C.Results on skin anti-inflammatory effect of complex hydrolysate from ginseng seed protein 47
1.NO(Nitric oxide) production inhibition assay 47
2.Anti-inflammatory test of RAW 264.7 cell via proteomic analysis 49
IV.CONCLUSION 61
REFERENCE 62
