3D printing of cartilage scaffold utilizing cartilage acellular matrix and silk fibroin
3D printing of cartilage scaffold utilizing cartilage acellular matrix and silk fibroin
- 주제(키워드) 3D printing , silk fibroin , cartilage acellular matrix
- 발행기관 아주대학교
- 지도교수 민병현
- 발행년도 2017
- 학위수여년월 2017. 2
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000024724
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Extracellular matrix (ECM) is known to provide instructive things to cell attachment, proliferation, differentiation ultimately tissue regeneration. Decellularized ECM scaffolds are rapidly expanding for regenerative medicine approaches. In this study, cartilage cellular matrix (CAM) biomaterial has been developed to fabricate of scaffolds. CAM provides adequate 3D support for the attachment, proliferation and differentiation of BMSCs into chondrocytes. Despite these important advantages in the tissue regeneration, CAM unable to precisely control pore size, interconnectivity and pore geometry in the scaffold because conventional protocols such as salt leaching, solvent casting, gas forming and freeze drying was used for manufacturing scaffolds. As an alternative to the scaffold fabrication method, 3D printing has recently been introduced in the field of tissue engineering. 3D printing could better control the internal microstructure and external appearance based on the computer-assisted delivery process. Hence, 3D printing technology is rapidly expanding for tissue engineering. However, ECM has to overcome some limitations, weak mechanical property, and rapid degradation for stable 3D structure stratification. Hence, we chose the silk fibroin to support the printing of CAM because silk fibroin can be crosslinked by physical stimulation as well as can be controlled viscosity easily. This study developed the hybrid printable composite for 3D printing with CAM and silk fibroin. The novel hybrid composite was printed by porous structure and evaluated the regenerative ability and printability for tissue regeneration.
more목차
Abstract--------------------------------------------------------------------------------------------ⅰ
Contents-------------------------------------------------------------------------------------------ⅱ
List of Figures------------------------------------------------------------------------------------ⅲ
1. Introduction-------------------------------------------------------------------------------------1
2. Materials and Methods-------------------------------------------------------------------------3
2.1 Preparation of biomaterials -------------------------------------------------------------3
2.2 Viscosity measurement------------------------------------------------------------------4
2.3 Fabrication of scaffolds-----------------------------------------------------------------4
2.4 Characterization of printed scaffold---------------------------------------------------5
2.5 Cell Seeding & chondrogenic differentiation----------------------------------------5
2.6 Cell compatibility------------------------------------------------------------------------6
2.7 Histological Analysis--------------------------------------------------------------------6
2.8 Statistical Analysis-----------------------------------------------------------------------6
3. Results-------------------------------------------------------------------------------------------7
3.1 Materials Characterization of printability test ---------------------------------------7
3.2 Morphological & mechanical characterizations-------------------------------------9
3.3 Degradation profile of 3D printed scaffolds ----------------------------------------11
3.4 Cell compatibility of printed scaffolds ----------------------------------------------13
3.5 Chondrogenesis of cells in Printed scaffolds----------------------------------------15
3.6 Mimetic scaffolds of the human articular cartilage --------------------------------17
4. Discussion-------------------------------------------------------------------------------------19
References-----------------------------------------------------------------------------------------21
국문요약-------------------------------------------------------------------------------------------23
