Triphasic scaffold fabrication with a barrier layer and its implications on osteochondral engineering and cartilage repair
- 주제(키워드) osteochondral scaffold , vascular invasion , cartilage repair
- 발행기관 아주대학교
- 지도교수 민병현
- 발행년도 2017
- 학위수여년월 2017. 2
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000024722
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Recent osteochondral scaffold strategies are required to mimic native tissue, which contains a calcified layer separated non-vascular cartilage tissue from bone tissue. It provides the optimal environment to individual tissue. One of these important roles of calcified layer is to inhibit an invasion of blood vessels from bone into cartilage. Vascular invasion could affect regenerated cartilage to be replaced with bone during repair. Therefore, it is required to prevent this process using a physical barrier in osteochondral scaffold. In this study, a triphasic scaffold with a barrier layer was developed. The barrier layer was designed to function to inhibit vascular invasion and improve cartilage repair as a calcified layer. The triphasic scaffold with a barrier layer was implanted into osteochondral defect of rabbit model. After 8 and 16 weeks, we observed that the triphasic scaffold with a barrier layer could inhibit the invasion of blood vessels from bone tissue into cartilage layer during development. However, stable hyaline cartilage failed to form. This research should be improved to investigate the implications of barrier layer after confirming to form stable cartilage first as a further work.
more목차
Abstract---------------------------------------------------------------------------------------------ⅰ
Contents--------------------------------------------------------------------------------------------ⅱ
List of Figures------------------------------------------------------------------------------------ⅲ
1. Introduction ------------------------------------------------------------------------------------1
2. Materials and Methods -----------------------------------------------------------------------3
2.1 Fabrication of osteochondral scaffolds -----------------------------------------------3
2.2 Migration of young rabbit chondrocytes labeled with PKH 26 in vitro ----------5
2.3 Histological observation of young rabbit chondrocytes induced to chondrogenic differentiation in CAM/Collagen scaffold -------------------------------------------------6
2.4 Scaffold implantation into osteochondral defect of rabbit knee -------------------7
2.5 Macroscopic observation of repaired cartilage --------------------------------------9
2.6 Microcomputed tomography observation of new bone formation ----------------9
2.7 Histological and Immunohistochemical analysis for observing repaired cartilage and vessel invasion ----------------------------------------------------------------------------9
3. Results -----------------------------------------------------------------------------------------10
3.1 Characterization of the osteochondral scaffolds ------------------------------------10
3.2 Migration of young rabbit chondrocytes labeled with PKH 26 in vitro ---------12
3.3 Histological observation of young rabbit chondrocytes chondrogenic differentiation induced in CAM/Collagen scaffold --------------------------------------14
3.4 Macroscopic evaluation of repaired cartilage --------------------------------------16
3.5 Histological and immunohistochemical evaluation of repaired cartilage and vessel invasion -------------------------------------------------------------------------------18
3.6 Micro CT evaluation of the subchondral bone formation -------------------------22
4. Discussion ------------------------------------------------------------------------------------24
References----------------------------------------------------------------------------------------26
국문요약------------------------------------------------------------------------------------------30
