Scaffold-free cartilage gel utilizing fetal cartilage-derived progenitor cells cultured on Nano-patterned substrate
- 주제(키워드) scaffold-free , aggregation , 3D culture
- 발행기관 아주대학교
- 지도교수 민병현
- 발행년도 2017
- 학위수여년월 2017. 2
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000024721
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
The microenvironment surrounding the cell is affected by the topography, mechanical, and chemical aspects of the surface. Among these, studies have been reported that cell spreading, differentiation, and cytoskeleton alignment are altered when the cellular microenvironment is controlled through topographic patterns. On the basis of the studies, many attempts have been made to use this pattered substrate as 3D culture methods of cells. 3D culture has the advantage of overcoming the limitation of the monolayer culture and replicating the in-vivo like environment of the cell. As an advantage of this 3D culture, many studies have been carried out to make cells into an aggregation form in order to re-differentiation of stem cells or to maintain phenotype. The aggregation method is particularly important in the chondrocyte that starts differentiation into the condensation phase of stem cells. In this study, we fabricated a 3D aggregation of cells utilizing fetal cartilage-derived progenitor cells (FCPCs), which are chondrogenic lineage cells and have a much higher proliferation and multi-lineage differentiation ability than chondrocyte and MSC, using Nano-patterned substrates. We manufactured aggregate pellet and this formation process was analyzed. These data demonstrate the potential of FCPCs three-dimensional aggregation using Nano-patterned substrate as an engineered cartilage gel.
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Abstract--------------------------------------------------------------------------------------------ⅰ
Contents--------------------------------------------------------------------------------------------ⅱ
List of Figures-----------------------------------------------------------------------------------ⅲ
1. Introduction-------------------------------------------------------------------------------------1
2. Materials and Methods-------------------------------------------------------------------------4
2.1 Isolation and culture of FCPCs---------------------------------------------------------4
2.2 Cell culture on Nano-patterned substrate----------------------------------------------4
2.3 Scanning electron microscope----------------------------------------------------------5
2.4 Immunocytochemistry-------------------------------------------------------------------5
2.5 Centrifuge adhesion analysis------------------------------------------------------------5
2.6 Aggregation assay------------------------------------------------------------------------5
2.7 Chondrogenic potential (Histology, μCT)---------------------------------------------6
2.8 Statistical analysis------------------------------------------------------------------------6
3. Results-------------------------------------------------------------------------------------------7
3.1 Aggregation formation of FCPCs by substrate coated Nano-particle size--------7
3.2 The Chondrogenic potential of 3D aggregates constructs of FCPCs using Nano-patterned substrate----------------------------------------------------------------------------11
3.3 Difference in cell attachment pattern of FCPCs on aggregation formation-----13
3.4 The role of cell-material adhesion molecules in FCPCs aggregation on various substrates---------------------------------------------------------------------------------------15
3.5 The role of cell-cell adhesion molecules in FCPCs aggregation on various substrates---------------------------------------------------------------------------------------17
4. Discussion-------------------------------------------------------------------------------------20
5. References-------------------------------------------------------------------------------------23
국문요약-------------------------------------------------------------------------------------------28
