Liquid type non-thermal plasma inhibits melanogenesis in human melanocyte
- 주제(키워드) Liquid type non-thermal plasma , melanocyte , melanogenesis
- 발행기관 아주대학교
- 지도교수 김철호
- 발행년도 2016
- 학위수여년월 2016. 8
- 학위명 석사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000023023
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Skin pigmentation is the result of abnormal production and deposition of melanin and exposure of skin tissue to UV causes the pigmentation. Although many studies had been performed to reduce skin pigmentation, there is no effective treatment without side effect has been developed. In this study, we demonstrated that Liquid type non-thermal Plasma (LTP) could inhibit melanogenesis effectively using in vivo and in vitro experiments. Using zebrafish model, we showed that LTP could decrease pigmentation. Treatment with LTP also inhibited melanin synthesis in foreskin tissue. The ability of LTP to decrease MITF and protein and mRNA level of tyrosinase that is a target gene of MITF was also demonstrated with primary melanocytes from donors. Constantly, treatment with LTP suppressed tyrosinase activity and melanin content. It is well known that Wnt signaling pathway is involved in the melanogenesis. Therefore, we determine the effect of LTP on activation of β-catenin. Treatment with LTP in melanocytes decreased active form of β-catenin, transcription factor LEF1/TCF4, MITF and tyrosinase protein levels in a time dependent manner. We also showed that expression of MITF and tyrosianse decreased using RT-PCR time dependently. In order to determine the translocation of active form of β-catenin into the nucleus, immunocytochemistry and Western blot with nuclear fraction were performed and the results showed that the level of active β-catenin and MITF decreased in the nucleus of LTP-treated cells compared with non-treat control cells. These results suggest that LTP down-regulates the melanogenesis and it might be a good candidate material for whitening cosmetics.
more목차
Ⅰ. INTRODUCTION 1
Ⅱ. MATERIAL AND METHOD 6
A. Experimental system specifications of NTP 6
B. Liquid type of plasma 7
C. Cell culture 8
D. Cell viability 9
E. Melanin content 10
F. Tyrosinase activity 10
G. Ex vivo skin organ culture and pigmentation assay in cultured skin 11
H. Immunocytochemistry 12
I. Western blotting 13
J. Reverse transcriptase-polymerase chain reaction (RT-PCR) 14
K. In vivo Zebrafish model 15
L. Statistical analysis 15
Ⅲ. RESULTS 17
1. LTP does not induce cytotoxicity in human melanocytes 17
2. LTP inhibits the pigmentation on zebrafish 18
3. LTP inhibits the pigmentation of cultured human skin 19
4. LTP inhibits melanin synthesis in primary melanocytes 20
5. LTP induces MITF downregulation 21
6. LTP reduces β-catenin in the nucleus 22
Ⅳ. DISCUSSION 32
Ⅴ. CONCLUSION 38
Ⅵ. REFERENCES 40
국문요약 51
