수면장애 모델에서 허브복합추추물과 그 성분인 linalool의 개선효과
Herbal mixture extract and its component ameliorate behavioral impairment induced by sleep disturbance
- 주제(키워드) Herb , Linalool , Sleep deprivation , Sleep disorder , Memory , Stress , Behavior
- 발행기관 아주대학교
- 지도교수 정이숙
- 발행년도 2016
- 학위수여년월 2016. 8
- 학위명 석사
- 학과 및 전공 일반대학원 약학
- 실제URI http://www.dcollection.net/handler/ajou/000000022869
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Herbal mixture extract is composed of Lavandula angustifolia, Chamaemeulum nobile, Aloysia triphylla, Thymus vulgaris, and Ocimum basilicum. Also, It has been reported that linalool, a major component of Lavandula angustifolia and Ocimum basilicum, has antioxidant, anti-inflammatory, neuroprotective effects, and so on. However, the effect of HME and linalool on behavior alterations induced by sleep deprivation has not been demonstrated yet. Several studies have demonstrated that sleep deprivation-induced memory impairment in animal models is well-known as a model of behavioral alterations. In the current study, we investigated the effects of HME (3, 10, 30, or 100 mg/kg, p.o.) and linalool (0.3, 1, or 3 mg/kg, i.p.) on cognitive and stress evaluation performance and biochemical parameters of sleep-disturbed mice. Spatial memory and learning memory were assessed by Y maze and passive avoidance test, respectively, and forced swimming test was also used to evaluate the anti-stress activity. After sleep deprivation induced by multiple platform method, HME and linalool significantly ameliorated spatial memory, learning memory, and stress activity. Linalool led to release serotonin, and reduce cortisol levels. In addition, HME resulted in release melatonin, and reduce cortisol concentrations in sleep disorder model. Our results suggest that HME and linalool have beneficial effects on behavioral alterations induced by sleep disturbance.
more목차
Ⅰ. INTRODUCTION 1
A. Importance of sleep 1
B. Insomnia 1
C. Herb 2
D. Linalool 3
E. Aims of study 3
Ⅱ. MATERIALS AND METHODS 4
A. Materials 4
B. Preparation of HME 4
C. Animals 4
D. Sleep disorder model 5
E. Sleep deprivation model 5
F. Sleep deprivation 6
G. Spontaneous alteration behavior Y maze test 6
H. Passive avoidance test 7
I. Forced swimming test 7
J. Plasma hormones concentrations 8
K. Statistical analysis 8
Ⅲ. RESULTS 9
A. The effect of the HME on memory impairment induced by sleep deprivation 9
B. The effect of the HME on stress induced by sleep deprivation 11
C. The effect of the HME on body weight in sleep deprivation moel 13
D. The effect of the HME on body weight in sleep disorder model 15
E. The effect of the HME on plasma melatonin in sleep disorder model 17
F. The effect of the HME on plasma cortisol in sleep disorder model 19
G. The effect of the linalool on spatial and working memory in sleep deprivation model 21
H. The effect of the linalool on learning memory in sleep deprivation model 23
I. The effect of the linalool on stress activity in sleep deprivation model 25
J. The effect of the linalool on serotonin level in sleep deprivation model 27
K. The effect of the linalool on cortisol level in sleep deprivation model 29
Ⅳ. DISCUSSION 31
Ⅴ. CONCLUSION 36
REFERENCES 37
국문요약 42
