목차

TABLE OF CONTENTSi
LIST OF FIGURES vi
LIST OF TABLES vii
ABBREVIATIONS viii
ABSTRACTxi
Chapter I 1
1. Intervertebral Disc (IVD) 2
1.1 Anatomy and development 2
1.2 Development 6
1.3 Cell population 8
1.4 Biochemical composition 11
1.5 Properties and function 17
1.6 Intervertebral disc degeneration and diseases 20
2. Tissue engineering for IVD regeneration 23
2.1 Tissue engineering 23
2.2 Cell source and strategy for IVD regeneration 24
2.2.1 Nucleus pulposus cells 25
2.2.2 Mesenchymal stem cells 26
2.3 Cell carriers for IVD regeneration 27
2.3.1 Fibrin and fibrinogen 28
2.3.2 Hyaluronic acid 29
2.4 Cell signal inducer for IVD regeneration 30
2.4.1 LIUS (low-intensity ultrasound) 30
3. Aim and title of this study 32
CHAPTER II Disc regeneration using autologous MSCs and fibrin/hyaluronic acid composite 33
1. Introduction 34
2. Materials and Methods 37
2.1 Isolation and culture of autologous mesenchymal stem cells 37
2.2 Preparation of fibrin and hyaluronic acid (Fi/HA) composite gel 37
2.3 Study design 38
2.4 Preparation of injured IVD model and materials transplantation 39
2.5 Magnetic resonance imaging 41
2.6 Macroscopic grading of IVD 42
2.7 Histological analysis 44
2.8 Statistical analysis 46
3. Result 47
3.1 Grades of disc degeneration by MRI 47
3.2 Gross evaluation 49
3.3 Histologic examination 53
4. Discussion 56
CHAPTER III Disc regeneration using Low-intensity ultrasound (LIUS) 60
1. Introduction 61
2. Materials and Methods 63
2.1 Study design 63
2.2 Preparation of injured intervertebral disc model 63
2.3 Treatment of Low-intensity ultrasound (LIUS) 66
2.4 Histological analysis 66
3. Results 67
3.1 Gross finding of intervertebral injury 67
3.2 Proteoglycan evaluation by safranin-O 69
3.3 Evaluation of collagen structure and deposition 69
4. Discussion 75
Chapter IV 78
REFERENCE 79
국문요약 88