The effects of a 1.8 GHz continuous electromagnetic fields on mucociliary transport of human nasal mucosa
1.8 GHz 전자기파가 인체 비점막의 점액 섬모 수송에 미치는 영향
- 주제(키워드) Electromagnetic fields , Ciliary motility , Mucociliary transport , Protein kinase C , Mobile phone
- 발행기관 아주대학교
- 지도교수 김현준
- 발행년도 2013
- 학위수여년월 2013. 2
- 학위명 박사
- 학과 및 전공 일반대학원 의학과
- 실제URI http://www.dcollection.net/handler/ajou/000000013577
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
The aim of this study was to investigate the effects of a 1.8 GHz continuous electromagnetic fields (EMF) on human nasal mucociliary transport and to determine the pathophysiology of ciliary beat frequency (CBF) during an EMF-induced change. Human nasal mucosa cells were exposed to a 1.8 GHz EMF (SAR= 1.0 W/kg), and CBF was analyzed using an optical flow technique with the peak detection method. The 1.8 GHz EMF-exposed group showed a decreased CBF when compared to the control group. In the cytotoxicity assay, the difference in survival rates was not found between the two groups. In the EMF-exposed group, protein kinase C (PKC) activity was increased during a PKC activity assay. The broad PKC inhibitor, Calphostin C abolished the EMF-induced decrease of CBF. The EMF-induced decrease of CBF was abolished by GF 109203X, a novel PKC (nPKC) isoform inhibitor, whereas the decrease was not attenuated by Gö-6976, a specific inhibitor of conventional PKC (cPKC) isoform. EMF may inhibit CBF via an nPKC-dependent mechanism. Therefore, we have confirmed that EMF could decrease CBF by increasing PKC activity.
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ABSTRACT
TABLE OF CONTENTS
LIST OF FIGURES
I. INTRODUCTION
II. MATERIAL AND METHODS
III.RESULTS
IV.DISCUSSION
V. CONCLUSION
REFERENCES
국문요약
