아주대학교

목차

List of Figures ⅰ
List of Tables ⅳ

Chapter Ⅰ. General introduction 1
Ⅰ.1. Definition and types of biodegradable polymer 2
Ⅰ.2. Characterization of biodegradable polymer for drug delivery system 4
Ⅰ.2.1. Aliphatic polyester 4
Ⅰ.2.2. Polyethylene glycol (PEG) 4
Ⅰ.2.3. Polycaprolactone (PCL) 4
Ⅰ.3. Injectable hydrogel for locally controlled drug delivery system 5
Ⅰ.4. Reference 8

Chapter Ⅱ. Injectable hydrogel based on biodegradable MPEG-b-(PCL-ran-PLLA) diblock copolymers for drug delivery system 10
Ⅱ.1. Introduction 11
Ⅱ.2. Experimental section 13
Ⅱ.2.1. Materials 13
Ⅱ.2.2. Characterization 13
Ⅱ.2.3. Synthesis of the MPEG-b-(PCL-ran-PLLA) diblock copolymer, C95L5 14
Ⅱ.2.4. Viscosity measurements 14
Ⅱ.2.5. In vivo injection 15
Ⅱ.2.6. Histological analysis 16
Ⅱ.2.7. Statistical analysis 17
Ⅱ.3. Results 18
Ⅱ.3.1. Synthesis and characterization of MPEG-b-(PCL-ran-PLLA) diblock copolymers 18
Ⅱ.3.2. Phase transition of diblock copolymer solutions 22
Ⅱ.3.3. In vitro incubation of diblock copolymer solutions at body temperature 24
Ⅱ.3.4. In vivo gelation and degradation 26
Ⅱ.3.5. In vivo immunogenicity of gels 28
Ⅱ.4. Discussion 31
Ⅱ.5. Conclusion 34

Chapter Ⅲ. Controlled delivery of antitumor agent from biocompatible and injectable in situ-forming gel based on MPEG-PCLA diblock copolymer 35
Ⅲ.1. Introduction 36
Ⅲ.2. Experimental section 38
Ⅲ.2.1. Synthesis of MPEG-PCL diblock copolymers 38
Ⅲ.2.2. Viscosity measurements 38
Ⅲ.2.3. In vitro Dox release 38
Ⅲ.2.4. Cytotoxicity studies 39
Ⅲ.2.5. In vivo tumor growth 40
Ⅲ.2.6. Histological analysis 41
Ⅲ.2.7. Statistical analysis 41
Ⅲ.3. Results 43
Ⅲ.3.1. Preparation of an injectable in situ-forming gel 43
Ⅲ.3.2. In vitro Dox release 45
Ⅲ.3.3. Antiproliferative effects of treatments 46
Ⅲ.3.4. Intratumoral injection 47
Ⅲ.3.5. Biodistribution of Dox 51
Ⅲ.3.6. Histology studies 54
Ⅲ.4. Discussion 57
Ⅲ.5. Conclusion 60

Reference 61
Abstract (in Korean) 68
List of publications 70