한국인 전신성 홍반성 루푸스에서 Fcγ 수용체 유전자들의 다형성
- 주제(키워드) Fcγ 수용체 , 다형성 , 전신성 홍반성 루푸스
- 발행기관 아주대학교
- 발행년도 2010
- 학위수여년월 2010. 8
- 학위명 석사
- 학과 및 전공 일반대학원 의생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000010922
- 본문언어 한국어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
국문요약 ················································································· ⅰ
차 례 ················································································· ⅳ
그림차례 ················································································· ⅵ
표 차례 ················································································· ⅶ
Ⅰ. 서 론 ·················································································· 1
Ⅱ. 재료 및 방법 ········································································ 5
A. 대상 환자군의 선별과 genomic DNA 준비 ·································· 5
1. 루푸스 환자군과 대조군 ····················································· 5
2. 환자군과 대조군으로부터 채혈 ············································· 5
3. Genomic DNA 추출 ························································ 5
B. 단일염기다형성 탐색 ····························································· 5
1. Fcg 수용체 ⅡA 유전자의 전사조절 부위와 exon4 부위에서 다형성의 유전자형 탐색 ································································· 5
2. Fcg 수용체 ⅡB 유전자의 exon5 와 intorn5 부위에서 다형성의 유전자형 탐색 ············································································· 6
C. 단일염기다형성의 유전자형 결정 ·············································· 7
1. Fcg 수용체 ⅡA 유전자의 전사조절 부위와 exon4, 그리고 Fcg 수용체 ⅡB 유전자의 exon5와 intorn5 부위에서 다형성의 유전자형 결정 ···· 7
E. 통계분석 ··········································································· 8
Ⅲ. 결 과 ················································································· 9
A. Fcg 수용체 ⅡA 유전자와 Fcg 수용체 ⅡB 유전자의 다형성 확인 ········ 9
1. Fcg 수용체 ⅡA 유전자의 전사조절 부위와 exon4 부위에서 다형성의 유전자형 확인 ··································································· 9
2. Fcg 수용체 ⅡB 유전자의 exon5 부위와 intron5 부위에서 다형성의 유전자형 확인 ································································· 10
3. Fcg 수용체 ⅡA 유전자와 Fcg 수용체 ⅡB 유전자의 다형성 통계분석 ·················································································· 10
B. 다형성의 유전자형 결정 ······················································· 11
1. Fcg 수용체 ⅡB 유전자의 exon5 부위와 intron5 부위에서 다형성의 유전자형 결정 ································································ 11
C. 루푸스 환자군과 정상 대조군의 임상적 특징 ································· 15
D. Fcg 수용체 ⅡB 유전자의 단일염기다형성의 유전자형과 임상적 특징과의 상관관계 ·········································································· 17
Ⅳ. 고 찰 ················································································· 19
Ⅴ. 결 론 ················································································ 22
참고 문헌 ················································································ 23
영문요약 ·················································································· 30
