인체 편평상피 세포암 조직에서 Nrf2 에 의존한 Sulfiredoxin 과 Peroxiredoxin III의 과 발현 양상과 임상적 의의
Nrf2-Dependent Overexpression of Sulfiredoxin and Peroxiredoxin III in Human Lung Cancer, Especially Squamous Cell Carcinoma
- 발행기관 아주대학교
- 발행년도 2010
- 학위수여년월 2010. 2
- 학위명 박사
- 학과 및 전공 일반대학원 의학과
- 실제URI http://www.dcollection.net/handler/ajou/000000010432
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
TABLE OF CONTENTS
ABSTRACT i
TABLE OF CONTENTS iii
LIST OF FIGURES v
ABBREVIATION vi
Ⅰ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 6
A. Materials 6
B. Subjects 6
C. Cell culture and viability 7
D. Transfection and depletion of siRNA 7
E. Immunocytochemistry 8
F. Co-immunoprecipitation and immunoblot analysis 8
G. Two-dimensional electrophoresis 9
H. Subcellular fractionation 10
I. Immunohistochemistry 10
J. Statistical analysis 11
Ⅲ. RESULTS 12
A. Localization of Prx III and Srx in A549 cells 12
B. Oxidation of Prx III in mink lung epithelial cells 12
C. Srx dependent regulation of Prx III oxidation in A549 cells 15
D. Srx is translocated into the mitochondria under oxidative stress 15
E. Overexpression of Prx I and III isoforms in human lung cancer 19
F. Nrf2 dependent expression of ARE-proteins in human lung cancer 19
G. Overexpression of Srx in human lung cancer 19
H. Survival of patients with lung cancer based on Srx expression 26
I. Expression of Srx in human lung cancer tissue 26
Ⅳ. DISCUSSION 28
Ⅴ. CONCLUSION 30
REFFERENCE 32
국문요약 40
