한외여과를 위한 다당류 층의 친수성 표면개질을 이용한 단백질 흡착제어
Control of protein adsorption using hydrophilic surface modification of polysaccharide layer for ultrafiltration
- 주제(키워드) protein , membrane , adsorption
- 발행기관 아주대학교
- 지도교수 이재의
- 발행년도 2010
- 학위수여년월 2010. 2
- 학위명 석사
- 학과 및 전공 일반대학원 응용생명공학과정
- 실제URI http://www.dcollection.net/handler/ajou/000000010347
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
This study makes to reduce a protein adsorption to the surface and obtain a high-yield product while we carry out the separation and extraction of protein. Adsorption of Protein, the most representative material among biomolecules, occurs at surface of membrane and disturbs efficient separation and extraction. To prevent the adsorption of protein, surface of membrane was modified into hydrophilic surface. In order to obtain hydrophilic surface, we used hydrophilic polysaccharide such as Pectin, Dextran, Hyaluronic acid and chitosan which is known a effective biomolecule's adsorption preventer. In order to enhance transmittance, we make membrane having a porous structure by using electrospinning. The modified surface of membrane was observed by SEM(Scanning Electron Microscope) and AFM(Atomic Force Microscope) and concentration amounts and adsorption amounts of protein were measured by BCA(Bicinchoninic acid) assay. And ultrafiltration system was used to investigate water flow rate. And the hydrophilic group is introduced into polysaccharide layer. Through this study, the protein adsorption of surface coated by polysaccharide was affected by electrostatic property between protein and polysaccharide. And the higher hydrophilicity of surface coated by polysaccharide is, the lower adsorption ratio of protein is. Also the membrane introducing MPEG-COOH on chitosan layer have an excellent performance regarding prevention of protein adsorption. These results lead us to the conclusion that membrane of several types were prepared by introducing functional groups
more목차
감사의 글
Abstract
List of Figure
List of Table
Ⅰ. Introduction
Ⅱ. Theoretical Approach
A. Dextran
B. Pectin
C. Hyaluronic acid
D. Chitosan
E. Example regarding prevention of protein adsorption
F. Electrospinning
G. BCA(Bicinchoninic acid) assay
Ⅲ. Material and methods
A. Material
B. Attachment of polysaccharide on film
C. Adsorption of protein on surface
D. Analysis
E. Preparation of PVA membrane
F. Measurement of concentration amounts and adsorption amounts
Ⅳ. Results and discussions
A. Fluorescence Intensity for BSA quantitative analysis
B. Data analysis using Isoelectric point
C. Data analysis using structure of hydrogel
D. Hystogram regarding protein adsorption distribution
E. Preparation of PVA membrane
F. Polysaccharide coating on membrane surface
G. Relation between the concentration of solution and layer thickness
H. Measurement of water flow rate
I. Measurement of concentration amount and adsorption amount
J. Preparation of polysaccharide layer introduced hydrophilic branch
1 Carboxyl-terminated PEG synthesis
2 Surface modification of complex polysaccharide layer
3. Estimation of adsorption ability for modified polysaccharide layer
Ⅴ. Conclusion
Reference
국문요약
