중뇌동맥을 결찰한 흰쥐의 산화망간조영증강 자기공명영상(MONERI) 연구
목차
국문요약 ⅰ
차례 ⅲ
그림 차례 ⅳ
표 차례 ⅵ
Ⅰ. 서론 1
Ⅱ. 연구대상 및 방법 4
A. 연구대상 4
B. 연구방법 6
1. 산화망간 나노입자 조영제 6
2. 영구폐색 국소 뇌허혈 동물모델 8
3. 산화망간 나노입자 조영제를 이용한 자기공명영상 10
4. 시간에 따른 신호 대 잡음비의 측정 11
5. H & E 염색 13
6. TUNEL 염색 13
7. 통계분석 14
Ⅲ. 결과 15
A. 중뇌동맥 폐색 후 흰쥐의 MONEMRI 영상소견 15
B. 뇌경색 이후 각 영역별 신호 대 잡음비의 시간에 따른 변화 25
C. 뇌손상 조직의 H&E 염색표본과 MR영상 비교 28
D. 뇌손상 조직의 TUNEL 염색표본과 MONEMRI 비교 32
Ⅳ. 고찰 35
Ⅴ. 결론 38
참고문헌 39
ABSTRACT 44
목차
그림 차례
Fig. 1. Schematic diagram of study protocol 5
Fig. 2. A manufacturing process of MnO nanoparticle 7
Fig. 3. Anatomy structure of cerebral artery in rat brain 9
Fig. 4. Localization of selected ROIs for SNR measurement
12
Fig. 5. Coronal T1 images at pre-occlusion, 3hrs, 6hrs,
24hrs, 72hrs and 1wk after the occlusion of right MCA
in rat 17
Fig. 6. Coronal T2 images at pre-occlusion, 3hrs, 6hrs,
24hrs, 72hrs and 1wk after the occlusion of right MCA
in rat 18
Fig. 7. Coronal diffusion-weighted images(DWI) at pre-
occlusion, 3hrs, 6hrs, 24hrs, 72hrs and 1wk after the
occlusion of right MCA in rat 19
Fig. 8. Coronal T2* images at pre-occlusion, 3hrs, 6hrs,
24hrs, 72hrs and 1wk after the occlusion of right MCA
in rat 20
Fig. 9. Coronal MnO enhanced T1 images at pre-
occlusion, 3hrs, 6hrs, 24hrs, 72hrs and 1wk after the
occlusion of right MCA in rat 21
Fig. 10. Coronal T2 images of MONEMRI at pre-occlusion,
3hrs, 6hrs, 24hrs, 72hrs and 1wk after the occlusion
of right MCA in rat 22
Fig. 11. Coronal DWI of MONEMRI at pre-occlusion, 3hrs,
6hrs, 24hrs, 72hrs and 1wk after the occlusion of
right MCA in rat 23
Fig. 12. Coronal T2* of MONEMRI at pre-occlusion, 3hrs,
6hrs, 24hrs, 72hrs and 1wk after the occlusion of
right MCA in rat 24
Fig. 13. Serial SNR of cerebral cortex, basal ganglia and
peripheral zone of infarcted area 26
Fig. 14. Comparing of coronal MnO enhanced T1 image and
corresponding H&E stained slide in rat brains after
3hrs, 6hrs, 24hrs, 72hrs and 1wk 29
Fig. 15. Hematoxylin and eosin staining of the ischemic
injure brain 31
Fig. 16. In situ detection of apoptoic cell death after MCA
occlusion 33
Fig. 17. High magnification of TUNEL positive cell. 34
