Ndrg-1의 하인두암에서의 발현과 임상적 의의
Expression and Clinical Significance of the N-myc downstream regulated gene-1 in Hypopharyngeal Cancer
- 주제(키워드) Hypopharyngeal , clinical , Ndrg-1 , expression
- 발행기관 아주대학교
- 지도교수 김철호
- 발행년도 2009
- 학위수여년월 2009. 2
- 학위명 석사
- 학과 및 전공 일반대학원 의학과
- 실제URI http://www.dcollection.net/handler/ajou/000000009815
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Expression and Clinical Significance of The N-myc downstream regulated gene-1 in Hypopharyngeal Cancer Objective. N-myc downstream regulated gene-1 (Ndrg-1), a potent metastasis suppressor gene, is identified as a gene that is up-regulated by the induction of differentiation in various cancer cell lines. Ndrg-1 has been shown to be involved in tumor progression and metastasis in colon, prostate and breast cancer. However, the clinical significance of Ndrg-1 in hypopharyngeal cancer remains to be investigated, and we therefore examined the role of Ndrg-1 in the survival rate, invasion and metastasis of hypopharyngeal squamous cell carcinoma (SCC). Materials and methods. We performed immunohistochemical staining of 56 specimens each of normal mucosa and SCC of the hypopharynx with Ndrg-1 antibodies. For reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting, fresh normal and cancer tissue from the hypopharynx obtained from three patients were used. Results. Positive rates of Ndrg-1 expression in hypopharyngeal SCC were 21.4%, respectively. Ndrg-1 staining was significantly inverse correlated with lymph node metastasis. (p=0.048) Decreased expression of Ndrg-1 protein (Western blotting) was detected in hypopharyngeal cancer tissue. Conclusion. Our results suggest that N-myc downstream regulated gene-1 (Ndrg-1) may play an important role in the progression and metastasis of hypopharyngeal cancer.
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TABLE OF CONTENTS
ABSTRACT i
TABLE OF CONTENTS ii
LIST OF FIGURES iii
LIST OF TABLES iv
Ⅰ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 3
A. Patients 3
B. Immunohistochemistry 3
C. Reverse transcriptase polymerase chain reaction 4
D. Western blotting 5
Ⅲ. RESULTS 6
A. Expression of Ndrg-1 in hypopharyngeal cancer tissue 6
B. RT-PCR and Western blotting in hypopharyngeal cancer tissue 9
Ⅳ. DISCUSSION 11
Ⅴ. CONCLUSION 16
REFERENCES 17
국문요약 21
