아주대학교

목차

ABSTRACT
TABLE OF CONTENTS
LIST OF FIGURE
LIST OF TABLES
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
A. Antibodies and reagents
B. Animal experiments
C. BD-like symptoms
D. Flow cytometry
E. Splenocyte culture and generation of Treg cells
F. Dendritic cells isolation and culture
G. Co-culture of CD4+T cells with DCs
H. Isolation of CD4+CD25+T cells
I.Adoptive transfer of CD4+CD25+T and CD4+CD25-T
cells to BD-like mice
J. ELISA
K. Reverse transcription PCR (RT-PCR)
L. Statistical analysis
Ⅲ. RESULTS
A. CD4+CD25+ Treg cells in BD-like mice compared to BDN mice
B. CD4+CD25+T cells were amplified in primary cultures of spleen tissues
C. CD4+T cells from normal, BDN, and BD-like splenocytes were co-cultured with normal dendritic cells from normal mice
D. Adoptive transfer with CD4+CD25+T cells up-regulated the frequencies of T cell in BD-like mice
E. Change of symptoms in BD-like mice after adoptive transfer
F. The change of severity score in transferred with CD4+CD25+T cells to BD-like mice
G. CD4+CD25+T cells transfer up-regulated serum IL-10 and TGF-β levels and down-regulated IFN-γ and TNF-α levels
H. Serum IL-6 and IL-17 levels were down-regulated after transfer
I. The differences of serum cytokine amounts between the average of not transferred BD-like mice and 3x10^5 CD4+CD25+T cells transferred each BD-like mouse
Ⅳ. DISCUSSION
Ⅴ. CONCLUSION
REFERENCES
국문요약