쥐의 뇌경색 모델에서 성인 중간엽 줄기세포 주입 후 신경생성 효과에 미치는 요인
Factors affecting neurogenesis after application of human mesenchymal stem cells in ischemic rat model
- 주제(키워드) human mesenchymal stem cell+stroke+neurogenesis+passage
- 발행기관 아주대학교
- 지도교수 허 균
- 발행년도 2006
- 학위수여년월 2006. 2
- 학위명 석사
- 학과 및 전공 일반대학원 의학과
- 본문언어 영어
초록/요약
Background: We recently reported the results of clinical trial of ex vivo culture expanded autologous mesenchymal stem cells (MSCs) in patients with severe ischemic stroke. We proposed that an optimal condition, for either donor or recipient, may exist and enhance the effects of the mesenchymal stem cells in those patients. Thus we evaluated the effect of the MSCs in impacts on the neurogenesis depending on the passage of MSCs. Methods and Results: We performed immunohistochemical studies and behavioral tests in transient middle cerebral occlusion rat model. Intravenous application of ex vivo cultured human mesenchymal cells, earlier (passage 2) or later (passage 6), was performed in stroke rat model. Compared to rat received later passage MSCs, neurogenesis revealed by bromodeoxyuridine staining was more pronounced in rat received early passage MSCs (P<0.05). Double staining showed that neuroblast (bromodeoxyuridine positive cells), but not MSCs (human nuclei matrix antigen positive cells), expressed neuronal and glial phenotypes. Conclusions: Our results indicated that ischemia-induced neurogenesis was enhanced by the application of mesenchymal stem cells, this process was related to the passage of ex vivo stem cell culture.
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TABLE OF CONTENTS
ABSTRACT ------------------------------------------- i
TABLE OF CONTENTS -------------------------------- iii
LIST OF FIGURES ------------------------------------- iv
LIST OF TABLES -------------------------------------- v
I. INTRODUCTION ------------------------------------- 1
II. MATERIALS AND METHODS ------------------------- 3
A. Animal MCAo model -------------------------------- 3
B. Experimental Groups -------------------------------- 3
C. hMSC culture --------------------------------------- 4
D. Cell baleling ---------------------------------------- 4
E. Functinal tests -------------------------------------- 5
F. Histological and immunohistochemical assessment -- 6
1. 2,3,5-triphenyltetrazolium chloride(TTC) staining ------ 6
2. Immunohistochemistry ------------------------------ 7
G. BrdU-labeled cell count in SVZ and ischemic border zone ------------------------------------------------- 9
III. RESULTS ----------------------------------------- 10
IV. DISCUSSION -------------------------------------- 12
REFERENCES ---------------------------------------- 22
국문요약 ----------------------------------------------27
