Preparation and Characterization of Functional Dyes and Bio Materials Molecular Films for Applications of Nanotechnology and Biotechnology
나노테크놀로지 와 바이오테크놀로지 응용을 위한 기능성 물질과 바이오물질을 사용한 기능성 분자박막 및 나노구조물 제조와 특성연구
- 발행기관 Department of Molecular Science and Technology Ajou University
- 지도교수 Kim, Jae-Ho
- 발행년도 2005
- 학위수여년월 2005. 2
- 학위명 박사
- 학과 및 전공 일반대학원 분자과학기술학과
- 본문언어 영어
초록/요약
This dissertation is related to applications for nanotechnology and biotechnology. In the concrete, this dissertation research is the fabrication and characterization of functionalized ultra thin film and nanostructures with functional dyes, such as hemicyanine dye, spirooxazine dye, and squarilym dye, diacetylene derivative, and biomaterials such as penicillinase and human immunoglobulin G for nanotechnology and biotechnology applications for example, molecular computer, molecular optical switch and memory, and biosensor. The dissertation was composed of 3 parts with 7 chapters, Part I is the introduction part and another parts were shown the research results. It can be divided into two parts according to the construction and characterization of molecular organic ultra-thin films with functional dyes and molecules in Chapter 2-5, while Chapter 6-7 are concentrated on the works with bio-molecules of enzymes as like as penicillinase and antibody-antigen as like as human immunoglobulin G. An outline of each chapter is as follows. Part Ⅰ is introduced the general background for understanding of the objects and published previous results in this dissertation. This part involves the introduction of each material, objects and published previous results of each research. Part Ⅱ is related to the fabrications and characterizations of functional dyes and molecules for nanotechnology applications. This part falls into four chapters as a kind of functionalized materials such as hemicyanine, spiroxazine, squrarilum dye, and diacetylene derivatives. Part Ⅲ was shown the constructions and characterizations of bio-materials for biotechnology applications. Enzymes and antigen-antibody were used for biomaterials such as penicillinase and human immunoglobulin G.
more초록/요약
본 논문은 나노테크놀로지와 바이오테크놀로지 응용을 위한 연구 결과들을 정리하였다. 구체적으로, 본 논문의 연구 결과는 분자소자, 분자 컴퓨터나 광학스위치나 메모리, 바이오센서등에 응용 가능한 hemicyanine dye, spiroxazine dye와 squarylim dye와 같은 기능성 염료 물질, diacetylene 물질과 같은 기능성 물질 및 penicillinase와 human immunoglobulin G와 같은 바이오 물질을 사용하여 기능성 박막이나 나노 구조체를 제작하고 이들의 특성을 연구하였다. 본 논문은 크게 3 Part로 나누어졌으며 총 9 Charts로 구성되었다. Part I의 개괄적인 소개와 연구 결과들로 정리하였다. 연구 결과는 chapter 4에서 7까지 기능성 염료와 기능성 물질을 사용한 유기 분자 박막의 제조 및 특성연구에 대한 부분과 chapter 8-9에서 효소와 항원-항체와 같은 생체물질을 사용하여 분자박막을 제조하고 이에 대한 특성을 연구한 부분으로 나누어 설명하였다. 각 부분의 연구 내용을 간단히 설명하면 다음과 같다. Part I은 본 논문을 목적과 중요성을 이해하는데 필요한 선행연구 결과와 연구 목적을 정리하였다. 이 부분에서는 각 물질 별로 선행연구 결과를 정리하고 이에 따라 본 연구에서 추구하는 목적에 대하여 정리하였다. Part II은 분자 스위치, 분자 메모리 소자, 분자 컴퓨터 등의 나노테크놀로지 분야에 응용 가능한 hemicyanine dye, spiroxazine dye와 squarylim dye와 같은 기능성 염료나 diacetylene과 같은 기능성 물질에 관한 연구 결과를 정리하였다. Part III은 대사작용이나 질병진단을 바이오센서에 응용 가능한 penicillinase와 같은 효소와 항원-항체 시스템인 human immunoglobulin G와 같은 바이오 물질을 사용하여 초박막의 제조 및 나노 구조체를 제조하고 이에 대한 특성을 평가한 연구 내용을 정리하였다.
more목차
TABLE OF CONTENTS
Title Page = ⅰ
Approval Page = ⅱ
Abstract = ⅲi
Table of Contents = ⅵ
List of Figures = xiii
List of Tables = xix
PART Ⅰ.Introcuction = 1
CHAPTER 1. Objects and Previous studies = 2
1.1. Hemicyanine dye = 3
1.2. Spiroxazine dyes = 8
1.3. Squarylium dye = 12
1.4. Diacetylene derivative = 13
1.5. Penicillinase = 16
1.6. Anti-human immunoglobulin G = 19
References = 21
PART Ⅱ.Fabrications and Characterization of Functional Dyes and Molecules = 37
CHAPTER 2. Hemicyanine Derivatives = 37
References = 39
2.1. Arachidic Acid as an Effector for Second Harmonic Generation Enhancement in Hemicyanine Langmuir-Blodgett Film = 40
Introduction = 40
Experimental Section = 42
Materials and Preparation of LB monolayers = 42
Characterization of LB monolayers = 44
Measurements of Molecular Orientation in Domains = 44
Results and Discussion = 45
AA as an Effector = 45
Characteristics of Domains = 54
Conclusions = 67
References = 68
2.2. Enhancement of for Second Harmonic Generation by Structural Changes of Amphiphilic Hemicyanine Monolayers through Perchlorate ions at Air-Water Interface = 73
Introduction = 73
Experimental = 74
Materials and Preparation of LB monolayers = 74
Characterization of LB monolayers = 76
Results and Discussion = 77
Effect of the salts in subphase = 77
Surface Potential-Area Isotherms of OHAPB on NaClO4 subphase = 82
AFM images of OHAPB LB monolayers = 83
Polarized UV-vis. absorption spectra of OHAPB = 89
in-situ vis. RAS of OHAPB = 90
FT-IR RAS and SERS of OHAPB = 95
SHG of OHAPB LB monolayers = 100
Conclusions = 101
References = 102
CHAPTER 3. Spiroxazine Derivatives = 105
References = 106
3.1. Preparation and Characterization of Photo-Switch Spiroxazine Self-Assembled Monolayer. = 108
Introduction = 108
Experimental Section = 110
Materials = 110
Preparation of self-assembled monolayer = 110
Characterization of self-assembled monolayer = 111
Results and Discussion = 112
Conclusions = 118
References = 120
3.2. Characterization of Quasi-Liquid Crystalline Spiroxazine Derivatives = 123
Introduction = 123
Experimental = 124
Materials = 124
Preparation of thin films = 124
Characterization of thin films = 125
Results and Discussion = 125
Conclusions = 131
References = 132
CHAPTER 4. Effects of Copper Cations in Subphase for Langmuir-Blodgett Monolayers of Squarylium Dyes = 133
Introduction = 133
Experimental = 135
Results and Discussion = 138
Monolayer Behavior on Cu subphase = 138
Conclusions = 153
References = 155
CHAPTER 5. Fabrication and Characterization of Diacetylene Derivative Langmuir and LB Monolayers before and after UV irradiation = 159
Introduction = 159
Experimental = 162
Materials = 162
Synthesis = 162
Preparation of LB monolayers = 163
Characterization of LB monolayers = 163
Results and Discussion = 166
Conclusions = 179
References = 180
PART Ⅲ. Fabrications and Characterization of Functionalized Molecular Thin Films with Biomaterials for Biosensor Applications = 184
CHAPTER 6. Immobilization of Penicillinase on Si3N4 Substrate by Carboxylic acid Terminated Coupling Layer for Penicillin-Sensitive LAPS Sensor = 184
Introduction = 184
Experimental = 187
Materials = 187
LAPS sensors = 188
Pre-treatments of Si3N4 = 188
Preparation of active coupling layer = 190
Penicillinase immobilization = 191
Results and Discussion = 191
pH response of LAPS device in standard pH buffer solution = 191
Immobilization of Penicillinase = 193
Penicillin response as coupling layer = 198
Conclusions = 200
References = 201
CHAPTER 7. Quantitative Analysis of HRP Labeled Immunogloblin G on Silicone Nitride Substrate by TMB = 208
Introduction = 208
Experimental = 209
Reagents = 209
Preparations of TMB solutions and Standard calibration curve = 212
Quantitative analysis for the amount of immobilized anti-hIgG = 212
Results and Discussion = 213
Conclusions = 217
References = 218
List of publications = 222
초록 = 231
