Isoalantolactone Suppresses Tumor Progression by regulating Hippo-YAP Signaling in Cholangiocarcinoma cell
- 주제(키워드) Cancer
- 주제(DDC) 570
- 발행기관 아주대학교 일반대학원
- 지도교수 Jung-Soon Mo
- 발행년도 2024
- 학위수여년월 2024. 2
- 학위명 석사
- 학과 및 전공 일반대학원 의생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000033733
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
I. Introduction 1
II. Material and Methods 5
1. Antibodies 5
2. Chemicals 5
3. Cell culture and transfection 5
4. CRISPR/Cas9 system 6
5. Retroviral infection and generation of stable cell lines 6
6. Cell lysis and Western blotting 7
7. MTT assay 7
8. Cell apoptosis assay 8
9. Immunoprecipitation (IP) 8
10. Immunocytochemistry 9
11. RNA isolation and qRT-PCR (quantitative real-time polymerase chain reaction) 9
12. Wound healing assay 10
13. Clonal growth assay 11
14. TA cloning 11
15. Tumor isolation from mouse 12
III. Results 13
1. Cytotoxic effect of IALT induces apoptosis in SNU478 cells 13
2. IALT regulates YAP activity via Hippo pathway 16
3. Absence of LATS1/2 counteracts the effect of IALT in regulating YAP 21
4. IALT inhibits Cell growth, Cell Migration and Tumorigenesis by controlling YAP activity 30
IV. Discussion 37
V. References 40
국문 초록 43