목차

1. Introduction 1
2. Experimental 7
2.1. Materials 7
2.2. Culturing human mesenchymal stem cells (hMSCs) in vitro 7
2.3. Preparation of fluorescence labeled hMSCs 8
2.4. Preparation of C-SIS and SP1 loaded C-SIS 9
2.5. Preparation of near-infrared (NIR) fluorescence-labeled SIS sponges 10
2.6. SEM analysis 11
2.7. Measurement of contact angle of SIS sponges 11
2.8. In vitro degradation assay of SIS sponges 12
2.9. In vitro peptide release assay of SP1 loaded SIS sponges 12
2.10. In vitro cytotoxicity test of SP and SP1 with hMSCs 13
2.11. In vitro migration of hMSCs with SP and SP1 14
2.12. In vitro migration of hMSCs with SP and SP1 loaded SIS sponges 14
2.13. Animal experiments 16
2.14. In vivo degradation assay of SIS sponges 16
2.15. In vivo peptide release assay of SP1 loaded SIS sponges 17
2.16. In vivo migration of ICG-labeled hMSCs toward SIS sponges 17
2.17. In vivo wound healing assay with SIS sponges 18
2.18. In vivo histological analysis by hematoxylin & eosin (H & E) staining / Masson trichrome staining (MTS) 19
2.19. In vivo immunofluorescence staining of migrated and vascularized hMSCs by CD29 / CD44 and CD31 staining 20
2.20. Statistical analysis 21
3. Results & Discussion 22
3.1. Characterization of manufactured SIS sponges 22
3.2. In vitro peptide release assay of SP1 loaded SIS sponges 25
3.3. In vitro cytotoxicity test of SP and SP1 with hMSCs 27
3.4. In vitro migration of hMSCs with SP and SP1 28
3.5. In vitro migration of hMSCs with SP and SP1 loaded SIS sponges 30
3.6. In vivo degradation assay of SIS sponges 33
3.7. In vivo peptide release assay of SP1 loaded SIS sponges 33
3.8. In vivo migration of ICG-labeled hMSCs toward SIS sponges 36
3.9. In vivo wound healing assay with SIS sponges 40
3.10. In vivo histological analysis by hematoxylin & eosin (H & E) staining / Masson trichrome staining (MTS) 43
3.11. In vivo immunofluorescence staining of migrated and vascularized hMSCs by CD29 / CD44 and CD31 staining 46
4. Conclusion 51
REFERENCES 52
LIST OF PUBLICATIONS 57
LIST OF AWARDS 59