Development of Injectable Demineralized- Bone and Hydroxyapatite-Nanoparticle Composite as a Bone Graft Material for Effective Healing of Rat Calvarial Defect
- 주제(키워드) bone graft , hydroxyapatite nanoparticle , demineralized bone matrix , injectable
- 주제(DDC) 547
- 발행기관 아주대학교 일반대학원
- 지도교수 Moon Suk Kim
- 발행년도 2024
- 학위수여년월 2024. 2
- 학위명 박사
- 학과 및 전공 일반대학원 분자과학기술학과
- 실제URI http://www.dcollection.net/handler/ajou/000000033268
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Demineralized bone matrix (DBM) stands out for its remarkable osteoconductive and osteoinductive properties, contributing to its efficacy in promoting bone healing and regeneration. However, it is prone to rapid resorption before substantial bone regeneration occurs. Our goal is to optimize an injectable formulation of DBM by integrating osteoconductive hydroxyapatite nanoparticles (HANP) to regulate resorption rates and enhance bone regeneration in bone defects. We formulated an injectable bone composite by mixed different ratio of DBM/HANP with varying concentrations of carboxymethyl cellulose (CMC) ranging from 5 wt% to 7 wt%, maintain a constant DBM/HANP:CMC as ratio 40:60 wt%. The viscoelasticity characteristic of the injectable composite increased with higher concentrations of CMC and DBM in the mixture. During syringe-based evaluations, DBM/HANP(50/50) with 5-7% CMC showed positive handling traits, including injectability, aggregation strength, composite adhesion, and shape retention. Additional extrusion tests showed higher extrusion pressures with increasing CMC concentrations. Based on these findings, we selected DBM/HANP(50/50)-5% CMC as the final injectable formulation. Subsequently, we compared the bone regeneration potential of DBM-5% CMC group and DBM/HANP(50/50)-5% CMC group in a skull defect site for 2 weeks and 8 weeks. Micro-CT, H&E stain, and Masson's trichrome stain (MTS) confirmed significantly enhanced new bone formation in the DBM/HANP(50/50)-5% CMC group compared to DBM-5% CMC. In conclusion, we effectively developed an injectable composite with an optimal blend of DBM and HANP, demonstrating good physical handling and injectability. Additionally, this formulation notably enhances bone healing in the surgery site.
more목차
BACKGROUND 1
1. Bone biology 1
2. Bone regeneration 3
3. Bone grafting 4
3.1 Optimal bone graft material 6
3.2 Classification of bone graft material 6
3.3 Bone graft carrier 15
I. INTRODUCTION 23
I-1. General introduction 23
I-2. The aim of the study 25
II. MATERIALS AND METHODS 26
II-1. Preparation of DBM 26
II-2. Radiological observation 26
II-3. ICP analysis 28
II-4. SEM observations 28
II-5. XRD analysis 28
II-6. FT-IR analysis 29
II-7. Thermogravimetric analysis (TGA) 29
II-8. Measurement of specific surface area 29
II-9. Preparation of DBM/HANP-CMC composites 30
II-10. Measurement of viscoelastic property 33
II-11. Extrusion pressure measurement 33
II-12. Evaluation of physical handling property 33
II-13. Surgical procedures 35
II-14. Micro-CT analysis 37
II-15. Histological staining and histomorphometric analysis 37
II-16. Statistical analysis 38
III. RESULTS AND DISCUSSION 39
III-1. Preparation and characterization of DBM 39
III-2. Chemical characterization of DBM/HANP mixtures 42
III-3. Evaluation of physical handling of injectable DBM/HANP-CMC composites 50
III-4. Evaluation of extrusion pressure of injectable DBM/HANP-CMC composites 56
III-5. Rheological characterization of injectable DBM/HANP-CMC composites 58
III-6. In vivo implantation of injectable DBM/HANP-CMC composites 61
III-7. Micro-CT analysis of bone regeneration 61
III-8. Histological analysis of bone regeneration 66
III-9. Histomorphometric analysis of bone regeneration 70
IV. CONCLUSION 72
REFERENCE 73
국문요약 85
