목차

1. Introduction 1
2. Experimental 6
2.1. Materials 6
2.2. Preparation of CP-loaded HA 7
2.3. Preparation of injectable hydrogel containing HA 8
2.4. Rheological properties of hydrogel 9
2.5. Injectability test of injectable formulations 10
2.6. Preparation of fluorescence labeled HA-CP 11
2.7. Preparation of fluorescence labeled PCLA 12
2.8. In vitro CP release and degradation of hydrogel 13
2.9. Synovial fibroblast isolation 14
2.10. In vitro cytotoxicity of hydrogel 15
2.11. Monitoring localization of CP from hydrogel 16
2.12. In vitro evaluation of pro-inflammatory cytokine level 17
2.13. In vivo CP release and degradation of hydrogel 18
2.14. Preparation of rat collagen-induced arthritis (CIA) model 19
2.15. In vivo therapeutic effects of PLP and PCP in CIA model 20
2.16. Histological assay 21
2.17. In vivo quantification of pro-inflammatory cytokines 22
2.18. Statistical analysis 23
3. Results & Discussion 24
3.1. Preparation and characterization of injectable formulations 24
3.2. In vitro CP release and degradation of hydrogel 28
3.3. In vitro evaluation of anti-inflammatory effect of CP from hydrogel 30
3.4. In vivo persistence of hydrogels in articular knee joint 34
3.5. Evaluation of inflammation relief via monitoring CIA model 36
3.6. Histological analysis of articular joint tissue 38
3.7. In vivo evaluation of pro-inflammatory cytokine level 40
4. Conclusion 42