목차

1. Introduction 1
1.1. Pancreatic cancer 1
1.2. Photodynamic therapy 2
1.3. Purpose of study 4
2. Materials and Methods 7
2.1. Materials 7
2.2 Cell line and culture condition 8
2.3 Phage amplification 8
2.4 Cell-based phage enzyme-linked immunosorbent assay (ELISA) 9
2.5 Phage labeling 9
2.6 Imaging of binding phages on cells 10
2.7 Conjugation of the pyropheophorbide-a to the selected phages 11
2.8 In vitro photodynamic therapy and cell viability assay 11
2.9 Animal models 12
2.10 In vivo fluorescence imaging of labeled phages 13
2.11 In vivo photodynamic therapy and tumor growth inhibition assay 13
2.12 Statistical analysis 14
3. Results and Discussion 14
3.1. Determination of apparent affinity of CBPs on pancreatic cancer cells through cell based ELISA 14
3.2 Phage labeling and imaging of binding phages on cells 16
3.3 In vivo fluorescence imaging of labeled phages 16
3.4 Preparation and characterization of PS conjugated phages 19
3.5 In vitro photodynamic therapy and cell viability assay 21
3.6 In vivo photodynamic therapy and tumor growth inhibition assay 23
4. Conclusion 26
References 27
국문요약 35