Expression and Purification of Engineered Recombinant Human Alpha-1 Antitrypsin for Collagen Targeting
- 주제(키워드) Alpha-1 antitrypsin , Collagen mimetic peptide , Recombinant protein , Collagen targeting , E. coli expression system
- 주제(DDC) 615.1
- 발행기관 아주대학교
- 지도교수 진효언
- 발행년도 2022
- 학위수여년월 2022. 8
- 학위명 석사
- 학과 및 전공 일반대학원 약학과
- 실제URI http://www.dcollection.net/handler/ajou/000000032185
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
목차
1. Introduction 1
2. Materials and Methods 7
2.1. Materials 7
2.2 Protein expression 7
2.3 Harvest cell lysates 8
2.4 1st purification by Ni-NTA affinity chromatography 8
2.5 2nd purification by using size-exclusion chromatography 9
2.6 Dialysis 9
2.7 BCA assay 9
2.8 SDS-PAGE 10
2.9 Determination of protein molecular weight using MALDI-TOF 10
2.10 Elastase inhibition activity of rhA1AT and rhA1AT-CMP 10
2.11 Collagen binding assay of rhA1AT and rhA1AT-CMP 11
3. Results 12
3.1 Purification of rhA1AT and rhA1AT-CMP 12
3.2 In vitro activity assay rhA1AT and rhA1AT-CMP 17
3.3 Analysis of binding properties of rhA1AT-CMP for collagen 19
4. Discussion 21
5. Conclusion 23
References 24
