Finding Markers for Stress Responses of Arabidopsis thaliana to Drought and Salt Stresses Using Metabolomics
Finding Markers for Stress Responses of Arabidopsis thaliana to Drought and Salt Stresses Using Metabolomics
- 주제(키워드) Arabidopsis thaliana , Stress Marker , Drought , Salt , Morphology , Flowering Time , Biomass , Plant Color , Metabolomics , GC-MS
- 발행기관 아주대학교
- 지도교수 박상규
- 발행년도 2020
- 학위수여년월 2020. 2
- 학위명 석사
- 학과 및 전공 일반대학원 생명과학과
- 실제URI http://www.dcollection.net/handler/ajou/000000029834
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
This study aimed to find water stress markers in Arabidopsis thaliana. I cultivated plants with drought and salt stresses in a growth room. To compare responses of each stress, I measured phenotypic traits first; then, I analyzed the metabolome pattern with gas chromatography-mass spectrometry (GC-MS). In morphology measurement, I considered three traits; flowering time, biomass, and plant color. Each morphology was analyzed using one-way ANOVA. Flowering time and biomass showed a tendency to reduction than the non-stress group. The color of plants divided by either drought or salt. Drought stress induces dark color, and salt stress presents a bright yellow color in Arabidopsis thaliana. Color changed patterns were elucidated with plant pigments using linear regression. In drought conditions, plant color was influenced by the accumulation of anthocyanin than reduction of chlorophyll a content and anthocyanin was more accumulated with strong stress states. However, in salt stress, plant color was influenced reduction of chlorophyll a than accumulation of anthocyanin. Unlike drought stress, chlorophyll a was only reduced significantly in harsh salt stress. It is hard to explain about stress level quantitatively. Thus, morphology measurement can be shown which stress is occurred to plants habitats, but it cannot explain stress level quantitatively. In metabolome analysis, I did a principal component analysis (PCA) for comparing metabolome pattern with each stress condition and finding stress markers. PCA result represented different metabolome patterns with stress strength in the same stress condition. It revealed different patterns by sort of stress as well. Then, I selected stress markers candidates using the loading values of each metabolite. Then, maltose and L-serine were selected for each water stress marker; drought stress marker is maltose and salt stress marker is L-serine. These specific markers play an important role as good indicators for each water stress in my model. As a result, specific metabolite markers can evaluate stress strength as well as identify each water stress. In conclusion, this study provides specific stress markers for water stress in Arabidopsis thaliana.
more목차
Ⅰ. Introduction 1
Ⅱ. Materials and methods 4
1. Cultivation and Stress Induction 4
2. Morphological phenotype measurement 5
2.1. Flowering time 7
2.2. Quantitative color comparing 7
2.3. Biomass 7
2.4. Estimation of plant pigments 9
2.4.1. Anthocyanin contents 9
2.4.2. Chlorophyll a contents 9
3. Metabolome profiling using GC-MS 10
3.1. Extraction of plant metabolites 10
3.2. Derivatization of metabolites 10
3.3. GC-MS condition 11
3.4. Data processing of GC-MS results 11
4. Test of stress identification and quantification using wild Arabidopsis thaliana 11
Ⅲ. Results 14
1. Various morphology of Arabidopsis thaliana for each stress 14
2. Pigments contents and color regression to stress levels 15
3. Metabolome changes in each stress and finding stress markers 23
4. Testing water stress markers to wild Arabidopsis thaliana 29
5. Making water stress model for evaluation water stress 29
Ⅳ. Discussion and Conclusion 34
Ⅴ. Reference 37
Ⅵ. Appendix 41
Ⅶ. 국문요약 42
