기도 상피 세포에서 Cleyera japonica에 의한 MUC5AC 억제 효능 및 기전
Effects of Cleyera japonica on MUC5AC expression and secretion in airway epithelial cells
- 주제(키워드) Cleyera japonica , Asthma , Respiratory inflammatory disease , Airway Epithelial Cells , NCI-H292 , Mucus , MUC5AC , MAPK , ERK , p38 , JNK
- 발행기관 아주대학교
- 지도교수 정이숙, 김수동
- 발행년도 2018
- 학위수여년월 2018. 2
- 학위명 석사
- 학과 및 전공 글로벌제약임상대학원 글로벌제약임상약학과
- 실제URI http://www.dcollection.net/handler/ajou/000000027601
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Cleyera japonica is known to inhibit inflammatory cytokines and to prevent or treat various allergic diseases by reducing or neutralizing allergenic proteins derived from house dust mite. Also, Plant extracts, including Cleyera japonica, provide compositions for the prevention or treatment of asthma. Based on this fact, we investigated whether Cleyera japonica considerably affects the gene expression and secretion of airway mucin. Confluent NCI-H292 cells were pretreated with Cleyera japonica for 30 min and then stimulated PMA (phorbol 12-myristate 13-acetate) for 24 h or the indicated periods. The MUC5AC gene expression was measured by RT-PCR. Secretion of MUC5AC protein were measured by ELISA. To elucidate the action mechanism of Cleyera japonica, effect of Cleyera japonica on PMA-induced MAPK signaling pathway was investigated by western blot analysis. The results were as follows: (1) Cleyera japonica inhibited the expression of MUC5AC protein induced by PMA; (2) Cleyera japonica inhibited the secretion of MUC5AC induced by PMA; (3) Cleyera japonica inhibited PMA-induced phosphorylation of p38, ERK and JNK. This result suggests that Cleyera japonica can regulate the gene expression, secretion of mucin by acting on airway epithelial cells via regulation of MAPK signaling pathway.
more목차
Ⅰ. INTRODUCTION 1
A. Asthma 1
B. Mucin overproduction in Asthma 1
C. Phorbol 12-myristate 13-acetate (PMA) and mitogen-activated protein kinase (MAPK) 2
D. Cleyera japonica 3
E. Aims of study 3
Ⅱ. MATERIALS AND METHODS 4
A. Materials 4
B. Cell culture 4
C. Treatment of cells with Cleyera japonica 5
D. MUC5AC mucin analysis using ELISA 5
E. RNA isolation and RT-PCR (reverse transcription- polymerase chain reaction) 6
F. Protein extraction and western blot analysis 6
G. Statistics 7
Ⅲ. RESULTS 8
A. PMA increased expression of MUC5AC in NCI-H292 cells 8
B. PMA increased the secretion of MUC5AC in NCI-H292 cells 10
C. Cleyera japonica inhibited PMA-induced expression of MUC5AC in NCIH 292 cells 10
D. Cleyera japonica inhibited PMA-induced MUC5AC secretion from NCI H292 cells 14
E. MAPK involved to PMA-induced MUC5AC expression in NCI-H292 cells 16
F. MAPK involved to PMA-induced MUC5AC secretion in NCI-H292 cells 18
G. PMA activated phosphorylation of ERK, p38, JNK MAPK in NCI-H 292 cells 20
H. Cleyera japonica inhibited PMA-induced activation of p38, ERK and JNK MAPK in NCI-H292 cells 22
IV. DISCUSSION 24
V. CONCLUSION 27
REFERENCES 28
국문요약 32
