집먼지 진드기에 의한 알레르기 비염 환자의 비점막에서 생성되는 국소 항체 및 염증 조절 매개체들과의 연관성
The local production of allergen-specific antibodies in nasal secretion from allergic rhinitis patients sensitive to D.farinae: Relationships with inflammatory mediators
- 주제(키워드) Allergic rhinitis , nasal mucosa , local specific antibody
- 발행기관 아주대학교
- 지도교수 박해심
- 발행년도 2017
- 학위수여년월 2017. 8
- 학위명 박사
- 학과 및 전공 일반대학원 의학과
- 실제URI http://www.dcollection.net/handler/ajou/000000025976
- 본문언어 영어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Background and Purpose: Allergic rhinitis (AR) is a common and increasing allergic disease, in which Dermatophagoides (D.) farinae is the most common causative allergen. The aims of this study are to compare locally produced antibodies to D. farinae in nasal mucosa between nasal provocation test (NPT)-positive and NPT-negative groups on NPT for D. farinae, to evaluate their correlation with the levels of inflammatory mediators, and to determine adaptive and innate immune responses in nasal mucosa. Subjects and Methods: Sixty AR patients underwent NPT for D. farinae. The sinus packs were placed in both nasal cavities of the patients for 5 minutes to obtain nasal secretion after NPT. The levels of total IgE, specific IgE to D. farinae, ECP, and tryptase were measured by using ImmunoCAP. Specific IgE, IgA to D. farina, and secretory IgA antibodies were measured by using ELISA. IL-8, VEGF, IL-25, and IL-33 levels were also measured by using ELISA Results: The high levels of total IgE, specific IgE, specific IgA, and secretory IgA, as well as inflammatory mediators, such as ECP, IL-8, VEGF and tryptase, were detected in nasal secretion, although the differences were not statistically significant between the NPT-positive and NPT-negative groups. The levels of total IgE, specific IgE to D. farinae, specific IgE, IgA to D. farina, and secretory IgA correlated with ECP (r=0.757, 0.436, 761, 0.737 and 0.692, respectively; all p<0.001). IL-33 and IL-25 were also highly detected, and IL-25 significantly correlated with IL-8 (r=0.625, p<0.001). Compared to the ELISA method, the ImmunoCAP system was more sensitive in the detection of specific IgE to D. farinae. Differences between right and left nasal secretions had no statistical significance. Conclusion: These findings confirmed the presence of locally produced specific antibodies to D. farinae in nasal secretion obtained from D. farinae-sensitive AR patients in which close correlations were noted between the antibodies and nasal inflammatory responses. It is suggested that both innate and adaptive immune responses may be involved in nasal inflammation of AR patients.
more목차
I. Introduction 1
II. Materials and Methods 3
A. MATERIALS 3
1. STUDY SUBJECTS 3
B. METHODS 3
1. Nasal provocation test 3
2. Sample collection and processing 4
3. Measurements of local antibodies, inflammatory mediators, and inflammatory markers in the nasal secretion samples 5
4. Calculation of concentrations and statistical analysis 6
5. Statistical analysis 6
III Results 8
A. Characteristics of the study subjects 8
B. Comparison of local antibody levels in nasal secretion between the NPT-positive and NPT-negative groups 10
C. Comparison of inflammatory markers in nasal secretion between the NPT-positive and NPT-negative groups 11
D. Comparison of IL-33 and IL-25 levels in nasal secretion between the NPT-positive and NPT-negative groups 12
E. Correlation between local specific antibodies to D. farinae and inflammatory markers in nasal secretion 12
F. Correlation of local specific antibodies and inflammatory mediators with IL- 25 and IL-33 17
IV. Discussion 19
V. Conclusion 25
References 26
국문요약 32
