풍선덩굴 추출물의 항염 항자극 효과연구
Studies on anti-inflammatory anti-irritation efficacies of Cardiospermum halicacabum Linn.
- 주제(키워드) Cardiospermum halicacabum Linn. , Apigenin , 자극완화 , 풍선덩굴
- 발행기관 아주대학교
- 지도교수 변상요
- 발행년도 2015
- 학위수여년월 2015. 8
- 학위명 석사
- 학과 및 전공 일반대학원 응용생명공학과정
- 실제URI http://www.dcollection.net/handler/ajou/000000020392
- 본문언어 한국어
- 저작권 아주대학교 논문은 저작권에 의해 보호받습니다.
초록/요약
Cardiospermum Halicacabum. was extracted with ethanol and fractionation by n-Hexane and EtOAc. This process have perfomed for getting Apigenin abundantly. Ethyl acetate fraction have 52% inhibition of Nitric oxide production efficacy. With same treating method on A-431 cells, Ethyle acetate fraction also had an anti-irritation efficacies. For application to cosmetic formulation, Silicagel chromathgraphy was performed to eliminate dark-green color of EtOAc fraction and enrich Apigenin concentration. As advantage of supplement in local Korea, Chamaecyparis obtusa. also studied for Anti-inflammation and Anti-irritation efficacies. Leaf and bark 1:1 ratio of Chamaecyparis obtusa. were extracted with n-Hexane for obtaining absolute oil. Absolute oil also have anti-inflammation efficacies and at 50 mg/L concentration, 45% of Nitric oxide production inhibition efficacies. From the human skin patch test, the two materials had 23.81% of anti irritation efficacies which shows that they had lower outcome than only Lactic Acid included formulation had. The results shows that, the following materials Cardiosperamum Halicacabum. and Chamaecyparis obtusa. have anti-inflammation and anti-irritation efficacies and the results explains that the two materials could be applied to cosmetics as an anti-inflammation and an anti-irritation material
more목차
국문 요약- i
목 차 -ii
표목차-iv
그림목차-v
1. Introduction-1
A. Cardiospermum Halicacabum.-1
1. Cardiospermum Halicacabum.-1
2. Apigenin-3
B. Chamaecypris obtusa.-4
C. Anti-inflammation-6
D. Irritation ( irritant contact dermatitis)-9
F. 연구 목적-12
2. Materials and Methods-13
A. Materials-13
B. Methods-13
1. Extraction and Fractionation-13
2. Purification-15
3. HPLC Analysis-15
4. Anti-inflammation and Anti-irritation efficacies-17
5. Cream formulation-18
6. Patch test-20
3. Results and Discussion
A. Extraction, Fractionation, Purification-21
1.Solvent Extraction-21
2.Optimization of extraction condition for Apigenin-22
3.Fractionation-23
4.Purification -24
B. HPLC Analysis -26
C. Anti-inflammation and Anti-irritation efficacies- 30
1.Cell cytotoxicity Test (MTT)- 30
2.Nitric oxide depression -32
3.Anti-irritation efficacies on A-431 Cell- 34
D. Application to Cosmetics -37
1.Patch test -37
4. Conclusion -38
5. Reference- 39
Abstract -42
