목차

Contents
List of Figures ⅰ
List of Tables ⅱ

Ⅰ. General Introduction 1
Ⅰ.1. Hydrogel 2
Ⅰ.2. Natural polymers 2
Ⅰ.3. Hydrogel prepared by electrostatic attraction 3

Ⅱ. Experiments 5
Ⅱ.1. Material selection 6
Ⅱ.1.1. Preparation of hydrogel 8
Ⅱ.1.2. In vivo hydrogel formation 9
Ⅱ.1.3. Histological analysis 9
Ⅱ.2. Injectable hydrogel prepared by electrostatic attraction 11
Ⅱ.2.1. Preparation of solution 12
Ⅱ.2.2. In vitro hydrogel formation 12
Ⅱ.2.3. Surface charge measurement 13
Ⅱ.2.4. In vivo hydrogel formation and histological analysis 13
Ⅱ.2.5. In vivo fluorescence imaging 14
Ⅱ.2.6. Biocompatibility of hydrogel 14
Ⅱ.2.7. Statistical analysis 15

Ⅲ. Results and discussion 16
Ⅲ.1. Material selection 17
Ⅲ.1.1. In vivo hydrogel formation 17
Ⅲ.1.2. Histological analysis 19
Ⅲ.2. Injectable hydrogel prepared by electrostatic attraction 22
Ⅲ.2.1. In vitro hydrogel formation 22
Ⅲ.2.2. In vivo hydrogel formation and histological analysis 25
Ⅲ.2.3. In vivo fluorescence imaging 27
Ⅲ.2.4. Biocompatibility of hydrogel 29

Ⅳ. Conclusion 30

Reference 32
Abstract 35
List of publications 37