자성체 나노 입자에 의한 세포 내 단백질 대사체 프로테아좀 유전자와 활성 변화 및 소포체 스트레스 분석
Analysis of Changes in Ubiquitin-Proteasome Pathway gene expression
초록/요약
Nanoparticles have been researched in various areas including energy, packaging, biotechnology, etc. because of the characteristics with size. The Magnetic Nanoparticles could be used for biological and medical purposes like drug delivery, NMR, MRI and sensors, so many studies have been researched. The purpose of the study are analyzing the proteasome activity of cells with MNPs@SiO2(RITC) and evaluating the effects. The microarray of the previous study showed the changes in gene level of proteasome. In high dose, genes of proteasome subunits were decreased about 39%, and genes of ubiquitin-proteasome were also changed. Based on these data, RT-PCR was used to confirm the changes of genes and the similar changes with microarray data were observed. With proteasome activity kit, it was observed that the proteasome activity was decreased by nanoparticles. And there are similar results with silica nanoparticles. Thus, it is supposed that effects of the MNPs@SiO2(RITC) were caused by the silica shell of the MNPs@SiO2(RITC). And GRP 78 protein level which is used the marker of ER stress was confirmed by western blot for confirming the effect to the ER stress of silica nanoparticles for further study. In other words, when using MNPs@SiO2(RITC), it could affect the proteasome activity and induce ER stress. Thus, this study suggests that MNPs@SiO2(RITC) can make some side effects to the proteasome activity and ER stress in the cell, but it is not very severe to launch the cell death. And further study has to be analyzed about ER stress with nanoparticles and proteasome inhibition.
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INTRODUCTION 1
MATERIALS AND METHODS 4
RESULTS 9
DISCUSSION 24
CONCLUSION 27
REFERENCES 28
ABSTRACT(KOREAN) 31
